Tested Applications
| Positive WB detected in | A431 cells, human brain tissue, Raji cells, HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | A431 cells, SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18322-1-AP targets MAD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13171 Product name: Recombinant human MAD1L1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 370-718 aa of BC009964 Sequence: QEELRQVSGQLLEERKKRETHEALARRLQKRVLLLTKERDGMRAILGSYDSELTPAEYSPQLTRRMREAEDMVQKVHSHSAEMEAQLSQALEELGGQKQRADMLEMELKMLKSQSSSAEQSFLFSREEADTLRLKVEELEGERSRLEEEKRMLEAQLERRALQGDYDQSRTKVLHMSLNPTSVARQRLREDHSQLQAECERLRGLLRAMERGGTVPADLEAAAASLPSSKEVAELKKQVESAELKNQRLKEVFQTKIQEFRKACYTLTGYQIDITTENQYRLTSLYAEHPGDCLIFKATSPSGSKMQLLETEFSHTVGELIEVHLRRQDSIPAFLSSLTLELFSRQTVA Predict reactive species |
| Full Name | MAD1 mitotic arrest deficient-like 1 (yeast) |
| Calculated Molecular Weight | 83 kDa |
| Observed Molecular Weight | 83 kDa |
| GenBank Accession Number | BC009964 |
| Gene Symbol | MAD1 |
| Gene ID (NCBI) | 8379 |
| RRID | AB_2139251 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6D9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MAD1L1 is a component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. It recruits MAD2L1 to unattached kinetochores. Also it is required for anchoring MAD2L1 to the nuclear periphery. In addition, it binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding [PMID:10049595, 12837246].
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MAD1 antibody 18322-1-AP | Download protocol |
| IP protocol for MAD1 antibody 18322-1-AP | Download protocol |
| WB protocol for MAD1 antibody 18322-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Sci Adv Biallelic germline mutations in MAD1L1 induce a syndrome of aneuploidy with high tumor susceptibility | ||
Cell Death Dis Genome-wide CRISPR screen identifies BUB1 kinase as a druggable vulnerability in malignant pleural mesothelioma | ||
Cancers (Basel) NFYA-Mediated TTK Up-Regulation Drives Fast Cell Cycle Progression and Its Inhibition Leads to Mitotic Catastrophe in Triple Negative Breast Cancer. | ||
BMB Rep TCP10L synergizes with MAD1 in transcriptional suppression and cell cycle arrest through mutual interaction. | ||
J Biol Chem N-acetyltransferase 10 promotes glioblastoma malignancy via mRNA stabilization of Jumonji and AT-rich interaction domain containing 2 |
