|Positive WB detected in||NIH/3T3 cells, MCF-7 cells, HeLa cells, mouse heart tissue, A2780 cells, CHO cells, SKOV-3 cells, K-562 cells, A375 cells, A431 cells, COLO 320 cells|
|Positive IHC detected in||human prostate cancer tissue, human brain tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Sample-dependent, check data in validation data gallery|
12330-2-AP targets TAK1 in WB, IP, IHC, ELISA applications and shows reactivity with human, hamster, mouse samples.
|Tested Reactivity||human, hamster, mouse|
|Cited Reactivity||human, Miiuy croaker, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TAK1 fusion protein Ag3041|
|Full Name||mitogen-activated protein kinase kinase kinase 7|
|Calculated molecular weight||579 aa, 64 kDa|
|Observed molecular weight||64-67 kDa, 75-80 kDa|
|GenBank accession number||BC017715|
|Gene ID (NCBI)||6885|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Transforming growth factor-β (TGF-β)-activated kinase 1 (TAK1), also known as MAP3K7 – mitogen-activated
protein kinase kinase kinase 7 – is a serine/threonine protein kinase triggering phosphorylation of AMP-activated
protein kinase (AMPK) and playing a role in the signal transduction of TGF-β, bone morphogenetic proteins
(BMPs), and various proinflammatory mediators.
What is the molecular weight of TAK1? Are there any isoforms of TAK1?
The molecular weight of full-length TAK1 is 55 kDa. There are four splicing variants of TAK1. TAK1-B represents
the full-length variant, while TAK1-A, TAK1-C, and TAK1-D lack exons 12 and/or 16. TAK-1 variants differ in their
expression levels across tissues (PMID: 11118615).
Is TAK1 post-translationally modified?
TAK1 kinase activity is modulated by interacting with TAK1-binding proteins (TABs). Binding to TABs triggers
autophosphorylation of TAK1 and depends on polyubiquitination of receptor-interacting protein 1 (RIP1)
(PMID: 8638164 and 11460167). Additionally, TAK1 can be ubiquitinated, acetylated, and O-glycosylated,
which impacts its activity (PMID: 28106845).
What is the subcellular localization of TAK1?
TAK1 is present in the cytoplasm.
What is the tissue expression pattern of TAK1?
TAK1 is ubiquitously expressed.
How can TAK1 activity be monitored?
TAK1 requires phosphorylation for its activity. TAK1 autophosphorylation in the presence of TBPs occurs on
Thr178, Thr184, Thr187, and Ser192 residues. The most commonly used phosphorylation readout is Thr187
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