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  • KD/KO Validated

MARCH8 Polyclonal antibody

MARCH8 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14119-1-AP

Synonyms

c MIR, MARCH VIII, MARCH8, MIR, RING finger protein 178, RNF178



Tested Applications

Positive WB detected inmouse testis tissue
Positive IP detected inmouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

14119-1-AP targets MARCH8 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MARCH8 fusion protein Ag5268
Full Name membrane-associated ring finger (C3HC4) 8
Calculated molecular weight 33 kDa
Observed molecular weight 30-33 kDa
GenBank accession numberBC066988
Gene symbol MARCH8
Gene ID (NCBI) 220972
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MARCH8 (Membrane-associated RING finger protein 8) is also named as MIR, RNF178 and belongs to the RING zinc finger protein family. It functions as an E3 ubiquitin ligase for immune recognition-related molecules (e.g. major histocompatibility complex class I, B7-2, and ICAM-1). It plays a key role in controlling MHC class II surface expression by regulated ubiquitination of a lysine residue in the beta-chain (PMID:22761441).

Protocols

Product Specific Protocols
WB protocol for MARCH8 antibody 14119-1-APDownload protocol
IP protocol for MARCH8 antibody 14119-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
zebrafishIHC

PLoS One

Cell adhesion in zebrafish embryos is modulated by march8.

Authors - Mi Ha Kim
humanWB

Oncogenesis

JWA regulates TRAIL-induced apoptosis via MARCH8-mediated DR4 ubiquitination in cisplatin-resistant gastric cancer cells.

Authors - Q Wang
  • KD Validated
humanWB

Proc Natl Acad Sci U S A

The E3 ubiquitin ligase MARCH8 negatively regulates IL-1β-induced NF-κB activation by targeting the IL1RAP coreceptor for ubiquitination and degradation.

Authors - Chen Rui R

Autophagy

BST2 suppresses porcine epidemic diarrhea virus replication by targeting and degrading virus nucleocapsid protein with selective autophagy.

Authors - Ning Kong
  • KD Validated
humanWB

Mol Cell

PMID: 28965816

Authors - Shouheng Jin
  • KO Validated