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MAX Polyclonal antibody

MAX Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, chIP,ELISA

Conjugate

Unconjugated

Cat no : 10426-1-AP

Synonyms

bHLHd4, bHLHd5, bHLHd6, bHLHd7, bHLHd8, MAX, MYC associated factor X, orf1, Protein max

Validation Data Gallery

  • WB analysis of HEK-293 using 10426-1-AP

    WB analysis of HEK-293 using 10426-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10426-1-AP (MAX Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 10426-1-AP

    IP experiment of HEK-293 using 10426-1-AP

    IP Result of anti-MAX (IP:10426-1-AP, 4ug; Detection:10426-1-AP 1:300) with HEK-293 cells lysate 1200ug.

  • IF Staining of PC-3 using 10426-1-AP

    IF Staining of PC-3 using 10426-1-AP

    Immunofluorescent analysis of PC-3 cells using 10426-1-AP (MAX antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, PC-3 cells, A549 cells
Positive IP detected inHEK-293 cells
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10426-1-AP targets MAX in WB, IP, IF, chIP,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAX fusion protein Ag0680
Full Name MYC associated factor X
Calculated molecular weight 18 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC003525
Gene symbol MAX
Gene ID (NCBI) 4149
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Max, a member of MAX family, contains a basic helix-loop-helix (bHLH) domain, is a transcription regulator. MAX can form a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, while the MAD-MAX complex is a repressor. MAX could dimerizated with another bHLH protein to form a heterodimer, such MYC or MAD. Thus bands recognized by this antibody much larger than predicted.

Protocols

Product Specific Protocols
WB protocol for MAX antibody 10426-1-APDownload protocol
IF protocol for MAX antibody 10426-1-APDownload protocol
IP protocol for MAX antibody 10426-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

NAD+ supplement potentiates tumor-killing function by rescuing defective TUB-mediated NAMPT transcription in tumor-infiltrated T cells.

Authors - Yuetong Wang
View Article
humanchIP

Sci Rep

Myc/Max dependent intronic long antisense noncoding RNA, EVA1A-AS, suppresses the expression of Myc/Max dependent anti-proliferating gene EVA1A in a U2 dependent manner.

Authors - Svenja E Niehus
View Article
humanchIP

J Biol Chem

Role for carbohydrate response element-binding protein (ChREBP) in high glucose-mediated repression of long noncoding RNA Tug1.

Authors - Jianyin Long
View Article
humanWB,ChIP

Elife

Loss of MGA repression mediated by an atypical polycomb complex promotes tumor progression and invasiveness.

Authors - Haritha Mathsyaraja
View Article
mouseWB

Cancer Cell

MAX Functions as a Tumor Suppressor and Rewires Metabolism in Small Cell Lung Cancer.

Authors - Arnaud Augert
  • KO Validated
View Article