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MAX Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 10426-1-AP

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Con: 55 μg/150 μl

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Tested Applications

Applications:
WB, IP, IF, ELISA
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Images See all

MAX Antibody WB HEK-293 cells 10426-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10426-1-AP( MAX Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

MAX Antibody IP HEK-293 cells 10426-1-AP

IP Result of anti-MAX (IP:10426-1-AP, 4ug; Detection:10426-1-AP 1:300) with HEK-293 cells lysate 1200ug.

MAX Antibody IF PC-3 cells 10426-1-AP

Immunofluorescent analysis of PC-3 cells using 10426-1-AP(MAX antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
HEK-293 cells, A549 cells, PC-3 cells
Positive IP detected in:
HEK-293 cells
Positive IF detected in:
PC-3 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
MAX fusion protein Ag0680
Full name:
MYC associated factor X
Calculated molecular weight:
18 kDa
Observed molecular weight:
22 kDa
GenBank accession number:
BC003525
Gene ID (NCBI):
4149
Gene symbol
MAX
Synonyms
bHLHd4, bHLHd5, bHLHd6, bHLHd7, bHLHd8, MAX, MYC associated factor X, orf1, Protein max

Background

Max, a member of MAX family, contains a basic helix-loop-helix (bHLH) domain, is a transcription regulator. MAX can form a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, while the MAD-MAX complex is a repressor. MAX could dimerizated with another bHLH protein to form a heterodimer, such MYC or MAD. Thus bands recognized by this antibody much larger than predicted.


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