MAX Polyclonal Antibody for IF, IP, WB, ELISA
Cat no : 10426-1-AP
bHLHd4, bHLHd5, bHLHd6, bHLHd7, bHLHd8, MAX, MYC associated factor X, orf1, Protein max
|Positive WB detected in||HEK-293 cells, PC-3 cells, A549 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IF detected in||PC-3 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10426-1-AP targets MAX in WB, IP, IF, chIP, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||MAX fusion protein Ag0680|
|Full Name||MYC associated factor X|
|Calculated molecular weight||18 kDa|
|Observed molecular weight||22 kDa|
|GenBank accession number||BC003525|
|Gene ID (NCBI)||4149|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Max, a member of MAX family, contains a basic helix-loop-helix (bHLH) domain, is a transcription regulator. MAX can form a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, while the MAD-MAX complex is a repressor. MAX could dimerizated with another bHLH protein to form a heterodimer, such MYC or MAD. Thus bands recognized by this antibody much larger than predicted.
Myc/Max dependent intronic long antisense noncoding RNA, EVA1A-AS, suppresses the expression of Myc/Max dependent anti-proliferating gene EVA1A in a U2 dependent manner.
J Biol Chem
Role for carbohydrate response element-binding protein (ChREBP) in high glucose-mediated repression of long noncoding RNA Tug1.