|Positive WB detected in||HepG2 cells, HEK-293 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human testis tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10685-1-AP targets MBIP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||MBIP fusion protein Ag1061|
|Full Name||MAP3K12 binding inhibitory protein 1|
|Calculated molecular weight||39 kDa|
|Observed molecular weight||39 kDa|
|GenBank accession number||BC005197|
|Gene ID (NCBI)||51562|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MBIP (MAP3K12 binding inhibitory protein 1), also known as MAPK upstream kinase (MUK)-binding inhibitory protein, is a 344 amino acid nuclear and cytoplasmic protein that assists in the activation of the JNK pathway by inhibiting ZPK activity. Ubiquitously expressed, MBIP is found at high levels in lung and heart and is one component of the ADA2A-containing complex (ATAC) in conjunction with ADA3, GCN5, CDC101, TADA2L, WDR5, YEATS2, DR1 and CRP2BP.
|Product Specific Protocols|
|WB protocol for MBIP antibody 10685-1-AP||Download protocol|
|IHC protocol for MBIP antibody 10685-1-AP||Download protocol|
|IF protocol for MBIP antibody 10685-1-AP||Download protocol|
|IP protocol for MBIP antibody 10685-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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