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MBIP Polyclonal antibody

MBIP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10685-1-AP

Synonyms

MBIP, MUK binding inhibitory protein



Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman testis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10685-1-AP targets MBIP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MBIP fusion protein Ag1061
Full Name MAP3K12 binding inhibitory protein 1
Calculated molecular weight 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC005197
Gene symbol MBIP
Gene ID (NCBI) 51562
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MBIP (MAP3K12 binding inhibitory protein 1), also known as MAPK upstream kinase (MUK)-binding inhibitory protein, is a 344 amino acid nuclear and cytoplasmic protein that assists in the activation of the JNK pathway by inhibiting ZPK activity. Ubiquitously expressed, MBIP is found at high levels in lung and heart and is one component of the ADA2A-containing complex (ATAC) in conjunction with ADA3, GCN5, CDC101, TADA2L, WDR5, YEATS2, DR1 and CRP2BP.

Protocols

Product Specific Protocols
WB protocol for MBIP antibody 10685-1-APDownload protocol
IHC protocol for MBIP antibody 10685-1-APDownload protocol
IF protocol for MBIP antibody 10685-1-APDownload protocol
IP protocol for MBIP antibody 10685-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Endoplasmic reticulum stress-responsive transcription factor ATF6α directs recruitment of the Mediator of RNA polymerase II transcription and multiple histone acetyltransferase complexes.

Authors - Sela Dotan D
humanWB

J Biol Chem

Human ATAC Is a GCN5/PCAF-containing acetylase complex with a novel NC2-like histone fold module that interacts with the TATA-binding protein.

Authors - Yuan-Liang Wang
humanWB

Mol Cell Biol

The double-histone-acetyltransferase complex ATAC is essential for mammalian development.

Authors - Guelman Sebastián S
HUMANWB

EMBO J

Distinct roles of GCN5/PCAF-mediated H3K9ac and CBP/p300-mediated H3K18/27ac in nuclear receptor transactivation.

Authors - Qihuang Jin
humanWB

Nature

Characterizing the cancer genome in lung adenocarcinoma.

Authors - Weir Barbara A BA
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