Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 16225-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 16225-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 16225-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 16225-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 16225-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 16225-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 16225-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 16225-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 16225-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 16225-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of Raji using 16225-1-AP
IP result of anti-MCL1 (IP:16225-1-AP, 3ug; Detection:16225-1-AP 1:1000) with Raji cells lysate 800ug.
IHC staining of human ovary cancer using 16225-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16225-1-AP (MCL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary cancer using 16225-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16225-1-AP (MCL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 16225-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MCL1 antibody (16225-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of Ramos using 16225-1-AP
1X10^6 Ramos cells were intracellularly stained with 0.5 ug Anti-Human MCL1 (16225-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Featured Product
KD/KO Validated

MCL1 Polyclonal antibody

MCL1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 16225-1-AP

Publications(146)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

Bcl2-L-3, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, EAT, Induced myeloid leukemia cell differentiation protein Mcl-1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (146)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, mouse spleen tissue, Raji cells, HL-60 cells, A431 cells, human liver tissue, HEK-293T cells, human brain tissue, K-562 cells, MCF-7 cells, HeLa cells
Positive IP detected in	Raji cells
Positive IHC detected in	human ovary cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells
Positive FC (Intra) detected in	Ramos cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 11 publications below
WB	See 128 publications below
IHC	See 15 publications below
IF	See 7 publications below
IP	See 6 publications below
CoIP	See 1 publications below

Product Information

16225-1-AP targets MCL1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MCL1 fusion protein Ag10397 Predict reactive species
Full Name	myeloid cell leukemia sequence 1 (BCL2-related)
Calculated Molecular Weight	350 aa, 37 kDa
Observed Molecular Weight	35-40 kDa
GenBank Accession Number	BC107735
Gene Symbol	MCL1
Gene ID (NCBI)	4170
RRID	AB_2143977
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q07820
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target. MCL-1 can be alternatively spliced into a long form (MCL-1L, 40 kDa) or a short form (MCL-1S, 30 kDa). (PMID: 15467463, PMID: 15147382)

Protocols

Product Specific Protocols
WB protocol for MCL1 antibody 16225-1-AP	Download protocol
IHC protocol for MCL1 antibody 16225-1-AP	Download protocol
IF protocol for MCL1 antibody 16225-1-AP	Download protocol
IP protocol for MCL1 antibody 16225-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell The mitochondrial DNAJC co-chaperone TCAIM reduces α-ketoglutarate dehydrogenase protein levels to regulate metabolism Authors - Wang Jiahui View Article
mouse	IHC	Nat Commun MCL-1 gains occur with high frequency in lung adenocarcinoma and can be targeted therapeutically. Authors - Enkhtsetseg Munkhbaatar View Article
human	WB	Nat Commun Small-molecule suppression of calpastatin degradation reduces neuropathology in models of Huntington's disease. Authors - Di Hu View Article
mouse	WB	Nat Commun IFN-β is a macrophage-derived effector cytokine facilitating the resolution of bacterial inflammation. Authors - Senthil Kumaran Satyanarayanan View Article
human	WB	EBioMedicine The LIV-1-GRPEL1 axis adjusts cell fate during anti-mitotic agent-damaged mitosis. Authors - Pingbo Chen View Article
human	WB,IP	Nat Commun USP9X regulates centrosome duplication and promotes breast carcinogenesis. Authors - Xin Li View Article