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- KD/KO Validated
MCT1 Polyclonal antibody
MCT1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 20139-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | BxPC-3 cells, Jurkat cells, HeLa cells, mouse brain tissue, mouse liver tissue, Ramos cells, HepG2 cells, Raji cells |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human colon cancer tissue, human cervical cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:3000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 8 publications below |
| IHC | See 4 publications below |
| IF | See 1 publications below |
Product Information
The immunogen of 20139-1-AP is MCT1 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | cow, human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MCT1 fusion protein Ag14098 |
| Full Name | solute carrier family 16, member 1 (monocarboxylic acid transporter 1) |
| Calculated molecular weight | 500 aa, 54 kDa |
| Observed molecular weight | 38-45 kDa |
| GenBank accession number | BC026317 |
| Gene symbol | SLC16A1 |
| Gene ID (NCBI) | 6566 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MCT1, the SLC16A1 gene product, is a trans-membrane symporter involved in lactate and pyruvate transportation. It plays an important role in lactic acid transport and H+ clearance in cancer cells. MCT overexpression had been observed in various cancers and may play an important role in tumorigenesis. Two isoforms of MCT1 exist due to the alternative splicing, with predicted MW of 54 kDa and 46 kDa, respectively. While western blot analysis detected MCT1 at an apparent molecular mass of 40-50 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MCT1 antibody 20139-1-AP | Download protocol |
| IHC protocol for MCT1 antibody 20139-1-AP | Download protocol |
| IF protocol for MCT1 antibody 20139-1-AP | Download protocol |
| IP protocol for MCT1 antibody 20139-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
FASEB J Tumoral microvesicle-activated glycometabolic reprogramming in fibroblasts promotes the progression of oral squamous cell carcinoma.
| ||
Sci Rep Acidic Stress Triggers Sodium-Coupled Bicarbonate Transport and Promotes Survival in A375 Human Melanoma Cells. | ||
J Anim Physiol Anim Nutr (Berl) Rno-miR-425-5p targets the DLST and SLC16A1 genes to reduce liver damage caused by excessive energy mobilization under cold stress. | ||
Oncol Rep Monocarboxylate transporter 1 is an independent prognostic factor in esophageal squamous cell carcinoma. | ||
J Cell Mol Med Low levels of AMPK promote epithelial-mesenchymal transition in lung cancer primarily through HDAC4- and HDAC5-mediated metabolic reprogramming. | ||
Exp Brain Res Lanthanum damages learning and memory and suppresses astrocyte-neuron lactate shuttle in rat hippocampus. |
