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MCT1 Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 20139-1-AP

Featured Product KD/KO validated

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Con: 44 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

MCT1 Antibody WB HeLa cells 20139-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 20139-1-AP( MCT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

MCT1 Antibody IP Raji cells 20139-1-AP

IP result of anti-MCT1(IP:20139-1-AP, 4ug; Detection:20139-1-AP 1:300) with Raji cells lysate 3600 ug

MCT1 Antibody IHC human breast cancer tissue 20139-1-AP

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 20139-1-AP( MCT1 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

MCT1 Antibody IHC human breast cancer tissue 20139-1-AP

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 20139-1-AP( MCT1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

MCT1 Antibody IF HeLa cells 20139-1-AP

Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 20139-1-AP (MCT1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, rat
Positive WB detected in:
HeLa cells, Raji cells, Ramos cells
Positive IP detected in:
Raji cells
Positive IHC detected in:
human breast cancer tissue, human cervical cancer tissue, human colon cancer tissue
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
MCT1 fusion protein Ag14098
Full name:
solute carrier family 16, member 1 (monocarboxylic acid transporter 1)
Calculated molecular weight:
500aa,54 kDa
Observed molecular weight:
38-45 kDa
GenBank accession number:
BC026317
Gene ID (NCBI):
6566
Gene symbol
SLC16A1
Synonyms
MCT, MCT 1, MCT1, Monocarboxylate transporter 1, SLC16A1

Background

MCT1, the SLC16A1 gene product, is a trans-membrane symporter involved in lactate and pyruvate transportation. It plays an important role in lactic acid transport and H+ clearance in cancer cells. MCT overexpression had been observed in various cancers and may play an important role in tumorigenesis. Two isoforms of MCT1 exist due to the alternative splicing, with predicted MW of 54 kDa and 46 kDa, respectively. While western blot analysis detected MCT1 at an apparent molecular mass of 40-50 kDa.


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