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  • KD/KO Validated

MCT1 Polyclonal antibody

MCT1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20139-1-AP

Synonyms

MCT, MCT 1, MCT1, Monocarboxylate transporter 1, SLC16A1



Tested Applications

Positive WB detected inBxPC-3 cells, Jurkat cells, HeLa cells, mouse brain tissue, mouse liver tissue, Ramos cells, HepG2 cells, Raji cells
Positive IP detected inRaji cells
Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20139-1-AP is MCT1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity cow, human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MCT1 fusion protein Ag14098
Full Name solute carrier family 16, member 1 (monocarboxylic acid transporter 1)
Calculated molecular weight 500 aa, 54 kDa
Observed molecular weight 38-45 kDa
GenBank accession numberBC026317
Gene symbol SLC16A1
Gene ID (NCBI) 6566
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MCT1, the SLC16A1 gene product, is a trans-membrane symporter involved in lactate and pyruvate transportation. It plays an important role in lactic acid transport and H+ clearance in cancer cells. MCT overexpression had been observed in various cancers and may play an important role in tumorigenesis. Two isoforms of MCT1 exist due to the alternative splicing, with predicted MW of 54 kDa and 46 kDa, respectively. While western blot analysis detected MCT1 at an apparent molecular mass of 40-50 kDa.

Protocols

Product Specific Protocols
WB protocol for MCT1 antibody 20139-1-APDownload protocol
IHC protocol for MCT1 antibody 20139-1-APDownload protocol
IF protocol for MCT1 antibody 20139-1-APDownload protocol
IP protocol for MCT1 antibody 20139-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

FASEB J

Tumoral microvesicle-activated glycometabolic reprogramming in fibroblasts promotes the progression of oral squamous cell carcinoma.

Authors - Erhui Jiang
  • KD Validated
humanWB

Sci Rep

Acidic Stress Triggers Sodium-Coupled Bicarbonate Transport and Promotes Survival in A375 Human Melanoma Cells.

Authors - Oscar C Y Yang
ratWB

J Anim Physiol Anim Nutr (Berl)

Rno-miR-425-5p targets the DLST and SLC16A1 genes to reduce liver damage caused by excessive energy mobilization under cold stress.

Authors - Limin Lang

Oncol Rep

Monocarboxylate transporter 1 is an independent prognostic factor in esophageal squamous cell carcinoma.

Authors - Xue Chen
humanWB

J Cell Mol Med

Low levels of AMPK promote epithelial-mesenchymal transition in lung cancer primarily through HDAC4- and HDAC5-mediated metabolic reprogramming.

Authors - Shoujie Feng
ratWB

Exp Brain Res

Lanthanum damages learning and memory and suppresses astrocyte-neuron lactate shuttle in rat hippocampus.

Authors - Cuihong Jin