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MCT1 Antibody 8 Publications

Rabbit Polyclonal| Catalog number: 20139-1-AP

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Con: 44 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

MCT1 Antibody WB HeLa cells 20139-1-AP

WB result of MCT1 antibody (20139-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCT1 transfected HeLa cells

MCT1 Antibody WB BxPC-3 cells 20139-1-AP

BxPC-3 cells were subjected to SDS PAGE followed by western blot with 20139-1-AP (MCT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

MCT1 Antibody IHC human colon cancer tissue 20139-1-AP

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:600 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

MCT1 Antibody IHC human colon cancer tissue 20139-1-AP

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:600 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

MCT1 Antibody IF HeLa cells 20139-1-AP

Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 20139-1-AP (MCT1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

MCT1 Antibody IP Raji cells 20139-1-AP

IP result of anti-MCT1(IP:20139-1-AP, 4ug; Detection:20139-1-AP 1:300) with Raji cells lysate 3600 ug

Species specificity:
human, mouse, cow, rat
Positive WB detected in:
BxPC-3 cells, HepG2 cells, Ramos cells, HeLa cells, mouse brain tissue, mouse liver tissue, Raji cells
Positive IP detected in:
Raji cells
Positive IHC detected in:
human colon cancer tissue, human breast cancer tissue, human cervical cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:2000-1:10000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:300-1:1200
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
MCT1 fusion protein Ag14098
Full name:
solute carrier family 16, member 1 (monocarboxylic acid transporter 1)
Calculated molecular weight:
500aa,54 kDa
Observed molecular weight:
38-45 kDa
GenBank accession number:
BC026317
Gene ID (NCBI):
6566
Gene symbol
SLC16A1
Synonyms
MCT, MCT 1, MCT1, Monocarboxylate transporter 1, SLC16A1

Background

MCT1, the SLC16A1 gene product, is a trans-membrane symporter involved in lactate and pyruvate transportation. It plays an important role in lactic acid transport and H+ clearance in cancer cells. MCT overexpression had been observed in various cancers and may play an important role in tumorigenesis. Two isoforms of MCT1 exist due to the alternative splicing, with predicted MW of 54 kDa and 46 kDa, respectively. While western blot analysis detected MCT1 at an apparent molecular mass of 40-50 kDa.


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