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MDM2 Antibody
MDM2 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IF, COIP, ELISA
Conjugate
Unconjugated
Cat no : 19058-1-AP
Synonyms
Double minute 2 protein, hdm2, HDMX, MDM2, Oncoprotein Mdm2, p53 binding protein Mdm2
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, MCF-7 cells, A549 cells, HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 34 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
19058-1-AP targets MDM2 in WB, IP, IF, COIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Reactivity | human, mouse, rat |
| Cited Species | Artemia sinica, human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | Mdm2 p53 binding protein homolog (mouse) |
| Calculated molecular weight | 55 kDa |
| Observed molecular weight | 30-55 kDa; 60-100 kDa |
| GenBank accession number | NM_002392 |
| Gene symbol | MDM2 |
| Gene ID (NCBI) | 4193 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
What is the cellular localization of MDM2?
MDM2 is predominantly localized in the nucleoplasm.
What is the tissue specificity of MDM2?
MDM2 is non-specific, expressing ubiquitously in the brain, heart, kidney, skin, and other tissues. Certain
isoforms only express in cancerous cells and not normal tissue.
With what other molecules does MDM2 interact?
Most notably, the oncogene protein MDM2 regulates transcription factor p53 by binding its transcriptional
activation domain and by E3-ubiquitin ligase activity. Ribosome proteins rpL5, rpL11, and rpL23 also bind to
MDM2, inhibiting E3 ubiquitin ligase activity and promoting the p53 pathway.
What competes with MDM2 activity?
Nutilin-3 was designed to compete with MDM2 in binding with p53, inducing activation of the p53 pathway.
What is the role of ubiquitination in MDM2 activity?
While p53 is also affected by other post-translational modifications, its stability is determined by ubiquitination
mediation of MDM2, suppressing p53 transcriptional regulation and leading to the proteosomal degradation of
p53.
How is MDM2 associated with cancer?
MDM2 inhibits the activity of the tumor-suppressive protein p53, which serves as an important factor in
promoting DNA repair or activating apoptotic pathways. Loss of p53 is associated with tumor development
and growth.
What is the human ortholog of MDM2?
Human double-minute type 2 (HDM2)
In what cellular circumstances does the p53-MDM2 complex dissociate?
Cellular stress or DNA damage result in the phosphorylation and activation of p53. Phosphorylation also
prevents p53-MDM2 interaction.
Where is the gene located?
Hdm2 is located on human 12q15.
Mdm2 is located on murine 10 D2.
PMIDs: 24309898, 29584707, 9840926, 28714398
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MDM2 antibody 19058-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Agric Food Chem Avenanthramide A induces cellular senescence via miR-129-3p/Pirh2/p53 signaling pathway to suppress colon cancer growth. | ||
J Enzyme Inhib Med Chem Design, synthesis and molecular mechanisms of novel dual inhibitors of heat shock protein 90/phosphoinositide 3-kinase alpha (Hsp90/PI3Kα) against cutaneous melanoma. | ||
Genes Cancer RASSF10 suppresses hepatocellular carcinoma growth by activating P53 signaling and methylation of RASSF10 is a docetaxel resistant marker. | ||
Oxid Med Cell Longev DpdtbA-Induced Growth Inhibition in Human Esophageal Cancer Cells Involved Inactivation of the p53/EGFR/AKT Pathway. | ||
Curr Med Sci Notch1 Drives the Formation and Proliferation of Intrahepatic Cholangiocarcinoma. | ||
Oncotarget Targeting P-glycoprotein function, p53 and energy metabolism: Combination of metformin and 2-deoxyglucose reverses the multidrug resistance of MCF-7/Dox cells to doxorubicin. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Wei (Verified Customer) (01-30-2020) | Weak band for mouse heart at basal state.
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