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MDM2 Antibody

MDM2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, COIP, ELISA

Conjugate

Unconjugated

Cat no : 19058-1-AP

Synonyms

Double minute 2 protein, hdm2, HDMX, MDM2, Oncoprotein Mdm2, p53 binding protein Mdm2



Tested Applications

Positive WB detected inA431 cells, MCF-7 cells, A549 cells, HUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

19058-1-AP targets MDM2 in WB, IP, IF, COIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species Artemia sinica, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name Mdm2 p53 binding protein homolog (mouse)
Calculated molecular weight 55 kDa
Observed molecular weight 30-55 kDa; 60-100 kDa
GenBank accession number NM_002392
Gene symbol MDM2
Gene ID (NCBI) 4193
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

What is the cellular localization of MDM2?

MDM2 is predominantly localized in the nucleoplasm.

What is the tissue specificity of MDM2?

MDM2 is non-specific, expressing ubiquitously in the brain, heart, kidney, skin, and other tissues. Certain 

isoforms only express in cancerous cells and not normal tissue.

With what other molecules does MDM2 interact?

Most notably, the oncogene protein MDM2 regulates transcription factor p53 by binding its transcriptional 

activation domain and by E3-ubiquitin ligase activity. Ribosome proteins rpL5, rpL11, and rpL23 also bind to 

MDM2, inhibiting E3 ubiquitin ligase activity and promoting the p53 pathway.

What competes with MDM2 activity?

Nutilin-3 was designed to compete with MDM2 in binding with p53, inducing activation of the p53 pathway.

What is the role of ubiquitination in MDM2 activity?

While p53 is also affected by other post-translational modifications, its stability is determined by ubiquitination 

mediation of MDM2, suppressing p53 transcriptional regulation and leading to the proteosomal degradation of 

p53.

How is MDM2 associated with cancer?

MDM2 inhibits the activity of the tumor-suppressive protein p53, which serves as an important factor in 

promoting DNA repair or activating apoptotic pathways. Loss of p53 is associated with tumor development 

and growth.

What is the human ortholog of MDM2?

Human double-minute type 2 (HDM2)

In what cellular circumstances does the p53-MDM2 complex dissociate?

Cellular stress or DNA damage result in the phosphorylation and activation of p53. Phosphorylation also 

prevents p53-MDM2 interaction.

Where is the gene located?

Hdm2 is located on human 12q15.

Mdm2 is located on murine 10 D2.

PMIDs: 24309898, 29584707, 9840926, 28714398


Protocols

Product Specific Protocols
WB protocol for MDM2 antibody 19058-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Agric Food Chem

Avenanthramide A induces cellular senescence via miR-129-3p/Pirh2/p53 signaling pathway to suppress colon cancer growth.

Authors - Rong Fu
humanWB

J Enzyme Inhib Med Chem

Design, synthesis and molecular mechanisms of novel dual inhibitors of heat shock protein 90/phosphoinositide 3-kinase alpha (Hsp90/PI3Kα) against cutaneous melanoma.

Authors - Feifei Qin
humanWB

Genes Cancer

RASSF10 suppresses hepatocellular carcinoma growth by activating P53 signaling and methylation of RASSF10 is a docetaxel resistant marker.

Authors - Yongshuai Jin
humanWB

Oxid Med Cell Longev

DpdtbA-Induced Growth Inhibition in Human Esophageal Cancer Cells Involved Inactivation of the p53/EGFR/AKT Pathway.

Authors - Zhuo Wang
human,mouseWB

Curr Med Sci

Notch1 Drives the Formation and Proliferation of Intrahepatic Cholangiocarcinoma.

Authors - Jun Guo
humanWB

Oncotarget

Targeting P-glycoprotein function, p53 and energy metabolism: Combination of metformin and 2-deoxyglucose reverses the multidrug resistance of MCF-7/Dox cells to doxorubicin.

Authors - Chaojun Xue

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wei (Verified Customer) (01-30-2020)

Weak band for mouse heart at basal state.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart
No image submitted with review