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ME1 Polyclonal antibody

Name: ME1 Polyclonal antibody
Brand: Proteintech
SKU: 16619-1-AP

ME1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16619-1-AP

Synonyms

HUMNDME, Malic enzyme 1, ME1, MES, NADP dependent malic enzyme, NADP ME

Validation Data Gallery

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AllWBIPIHC

WB analysis of mouse liver using 16619-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 16619-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 16619-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse placenta using 16619-1-AP
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16619-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16619-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16619-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16619-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16619-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 16619-1-AP
IP result of anti-ME1 (IP:16619-1-AP, 4ug; Detection:16619-1-AP 1:500) with mouse liver tissue lysate 4000ug.
IHC staining of human liver using 16619-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16619-1-AP (ME1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 16619-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16619-1-AP (ME1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human liver using 16619-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16619-1-AP (ME1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 16619-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16619-1-AP (ME1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human placenta using 16619-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 16619-1-AP (ME1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 16619-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 16619-1-AP (ME1 antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 16619-1-AP

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Tested Applications

Positive WB detected in	mouse liver tissue, HeLa cells, mouse placenta tissue, MCF-7 cells, rat liver tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human liver tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 17 publications below
IHC	See 6 publications below
IF	See 1 publications below

Product Information

16619-1-AP targets ME1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ME1 fusion protein Ag9916
Full Name	malic enzyme 1, NADP(+)-dependent, cytosolic
Calculated Molecular Weight	572 aa, 64 kDa
Observed Molecular Weight	55-64 kDa
GenBank Accession Number	BC025246
Gene Symbol	ME1
Gene ID (NCBI)	4199
RRID	AB_2143821
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ME1(NADP-dependent malic enzyme) belongs to the malic enzymes family. This malic enzyme catalyzes the reversible oxidative decarboxylation of malate and is a link between the glycolytic pathway and the citric acid cycle. The reaction is L-malate plus NADP(+) to form pyruvate, CO(2), and NADPH. The predicted protein contains 572 amino acids and has a calculated molecular mass of 64.1 kDa.

Protocols

Product Specific Protocols
WB protocol for ME1 antibody 16619-1-AP	Download protocol
IHC protocol for ME1 antibody 16619-1-AP	Download protocol
IP protocol for ME1 antibody 16619-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Cell Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming. Authors - Yoichiro Mitsuishi View Article
mouse	WB,IHC	Adv Sci (Weinh) Malic Enzyme 1 as a Novel Anti-Ferroptotic Regulator in Hepatic Ischemia/Reperfusion Injury Authors - Xuexian Fang View Article
		Mol Cell Dynamic Regulation of ME1 Phosphorylation and Acetylation Affects Lipid Metabolism and Colorectal Tumorigenesis. Authors - Yahui Zhu View Article
human	WB	Cell Res cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. Authors - Linchong Sun View Article
mouse	WB	Cell Rep Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism Authors - Hao Qin View Article
	WB	Cell Rep Inhibiting G6PD by quercetin promotes degradation of EGFR T790M mutation Authors - Zehe Ge View Article

ME1 Polyclonal antibody

ME1 Polyclonal Antibody for IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis of mouse liver using 16619-1-AP

WB analysis using 16619-1-AP

WB analysis using 16619-1-AP

WB analysis of mouse placenta using 16619-1-AP

WB analysis of mouse liver using 16619-1-AP

WB analysis of mouse liver using 16619-1-AP

WB analysis of mouse liver using 16619-1-AP

WB analysis of mouse liver using 16619-1-AP

WB analysis of mouse liver using 16619-1-AP

IP experiment of mouse liver using 16619-1-AP

IHC staining of human liver using 16619-1-AP

IHC staining of human liver using 16619-1-AP

IHC staining of human liver using 16619-1-AP

IHC staining of human liver using 16619-1-AP

IHC staining of human placenta using 16619-1-AP

IHC staining of human placenta using 16619-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming.

Malic Enzyme 1 as a Novel Anti-Ferroptotic Regulator in Hepatic Ischemia/Reperfusion Injury

Dynamic Regulation of ME1 Phosphorylation and Acetylation Affects Lipid Metabolism and Colorectal Tumorigenesis.

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism

Inhibiting G6PD by quercetin promotes degradation of EGFR T790M mutation