MME,CD10 Monoclonal antibody

MME,CD10 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

4B9D9

Cat no : 60034-3-Ig

Synonyms

Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11



Tested Applications

Positive WB detected inpig kidney tissue, mouse kidney tissue
Positive IHC detected inhuman renal cell carcinoma tissue, human kidney tissue, human tonsil tissue, mouse kidney tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

60034-3-Ig targets MME,CD10 in WB, IHC,ELISA applications and shows reactivity with human, pig, mouse samples.

Tested Reactivity human, pig, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MME,CD10 fusion protein Ag0427
Full Name membrane metallo-endopeptidase
Calculated molecular weight 750 aa, 86 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC101658
Gene symbol MME
Gene ID (NCBI) 4311
Conjugate Unconjugated
Form Liquid
Purification Method Caprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

Protocols

Product Specific Protocols
WB protocol for MME,CD10 antibody 60034-3-IgDownload protocol
IHC protocol for MME,CD10 antibody 60034-3-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols