Tested Applications
| Positive WB detected in | A549 cells, human colon tissue, mouse heart tissue |
| Positive IHC detected in | human kidney tissue, human ovary tissue, human skin cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18237-1-AP targets MMP28 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12980 Product name: Recombinant human MMP28 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 194-393 aa of BC002631 Sequence: LGNAFDGPGGALAHAFLPRRGEAHFDQDERWSLSRRRGRNLFVVLAHEIGHTLGLTHSPAPRALMAPYYKRLGRDALLSWDDVLAVQSLYGKPLGGSVAVQLPGKLFTDFETWDSYSPQGRRPETQGPKYCHSSFDAITVDRQQQLYIFKGSHFWEVAADGNVSEPRPLQERWVGLPPNIEAAAVSLNDGDFYFFKVQSV Predict reactive species |
| Full Name | matrix metallopeptidase 28 |
| Calculated Molecular Weight | 59 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC002631 |
| Gene Symbol | MMP28 |
| Gene ID (NCBI) | 79148 |
| RRID | AB_2146444 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H239 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MMP28(Epilysin)can degrade casein and play a role in tissues homeostasis and repair .The only specific human cell type recognized to express epilysin is the basal keratinocyte in the skin, where epilysin expression is upregulated during wound repair(PMID:16940349).Western blot showed this antibody recognize bands at 62 and 58 kDa as well as breakdown products at 50, 48, and 46 kDa(PMID:15953044).This antibody is specific to MMP28.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MMP28 antibody 18237-1-AP | Download protocol |
| IHC protocol for MMP28 antibody 18237-1-AP | Download protocol |
| WB protocol for MMP28 antibody 18237-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-MMP28 antibody (Cat# 18237-1-AP) has 15 citations published in journals including J Exp Clin Cancer Res, Biomaterials, Int J Biol Sci, Phytomedicine, Biochem Pharmacol, Cell Mol Life Sci, Liver Int, Am J Pathol, FASEB J, Dis Model Mech, JBMR Plus, Int J Ophthalmol, and Curr Eye Res. Research fields span pancreatic cancer, hepatocellular carcinoma, gastric cancer, diabetic nephropathy, ALS, emphysema, vascular aging, osteoarthritis, uveal melanoma, and pterygium.
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res Reciprocal regulation of MMP-28 and EGFR is required for sustaining proliferative signaling in PDAC | ||
Biomaterials Decoding vascular aging: Substrate stiffness and shear stress orchestrate endothelial inflammation and remodelling via mechanosensitive pathways. | ||
Int J Biol Sci Upregulated MMP28 in Hepatocellular Carcinoma Promotes Metastasis via Notch3 Signaling and Predicts Unfavorable Prognosis.
| ||
Phytomedicine Targeting the NF-κB p65-MMP28 axis: Wogonoside as a novel therapeutic agent for attenuating podocyte injury in diabetic nephropathy | ||
Biochem Pharmacol MMP28 promotes tumorigenesis and gemcitabine resistance in pancreatic cancer by promoting glycolysis through interaction with SND1.
| ||
Cell Mol Life Sci Microglial activation in an amyotrophic lateral sclerosis-like model caused by Ranbp2 loss and nucleocytoplasmic transport impairment in retinal ganglion neurons. |
