|Positive WB detected in||A549 cells, human colon tissue, mouse heart tissue|
|Positive IHC detected in||human kidney tissue, human ovary tissue, human skin cancer tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A549 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
18237-1-AP targets MMP28 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MMP28 fusion protein Ag12980|
|Full Name||matrix metallopeptidase 28|
|Calculated molecular weight||59 kDa|
|Observed molecular weight||48 kDa|
|GenBank accession number||BC002631|
|Gene ID (NCBI)||79148|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MMP28(Epilysin)can degrade casein and play a role in tissues homeostasis and repair .The only specific human cell type recognized to express epilysin is the basal keratinocyte in the skin, where epilysin expression is upregulated during wound repair(PMID:16940349).Western blot showed this antibody recognize bands at 62 and 58 kDa as well as breakdown products at 50, 48, and 46 kDa(PMID:15953044).This antibody is specific to MMP28.
Int J Biol Sci
Upregulated MMP28 in Hepatocellular Carcinoma Promotes Metastasis via Notch3 Signaling and Predicts Unfavorable Prognosis.
KLF9 suppresses gastric cancer cell invasion and metastasis through transcriptional inhibition of MMP28.
Am J Pathol
Matrix Metalloproteinase-28 Is a Key Contributor to Emphysema Pathogenesis.
Dis Model Mech
Loss of Ranbp2 in motor neurons causes the disruption of nucleocytoplasmic and chemokine signaling and proteostasis of hnRNPH3 and Mmp28, and the development of amyotrophic lateral sclerosis (ALS)-like syndromes.
Am J Pathol
CD133 affects the invasive ability of HCT116 cells by regulating TIMP-2.
Drastic induction of MMP-7 by cortisol in the human amnion: implications for membrane rupture at parturition.