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  • KD/KO Validated

MMP28 Polyclonal antibody

MMP28 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18237-1-AP

Synonyms

EPILYSIN, matrix metallopeptidase 28, Matrix metalloproteinase 28, MM28, MMP 28, MMP28



Tested Applications

Positive WB detected inA549 cells, human colon tissue, mouse heart tissue
Positive IHC detected inhuman kidney tissue, human ovary tissue, human skin cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

18237-1-AP targets MMP28 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMP28 fusion protein Ag12980
Full Name matrix metallopeptidase 28
Calculated molecular weight 59 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC002631
Gene symbol MMP28
Gene ID (NCBI) 79148
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MMP28(Epilysin)can degrade casein and play a role in tissues homeostasis and repair .The only specific human cell type recognized to express epilysin is the basal keratinocyte in the skin, where epilysin expression is upregulated during wound repair(PMID:16940349).Western blot showed this antibody recognize bands at 62 and 58 kDa as well as breakdown products at 50, 48, and 46 kDa(PMID:15953044).This antibody is specific to MMP28.

Protocols

Product Specific Protocols
WB protocol for MMP28 antibody 18237-1-APDownload protocol
IHC protocol for MMP28 antibody 18237-1-APDownload protocol
IF protocol for MMP28 antibody 18237-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Int J Biol Sci

Upregulated MMP28 in Hepatocellular Carcinoma Promotes Metastasis via Notch3 Signaling and Predicts Unfavorable Prognosis.

Authors - Jiangfan Zhou
  • KD Validated
humanWB

FASEB J

KLF9 suppresses gastric cancer cell invasion and metastasis through transcriptional inhibition of MMP28.

Authors - Yang Li
humanIHC

Am J Pathol

Matrix Metalloproteinase-28 Is a Key Contributor to Emphysema Pathogenesis.

Authors - Anne M Manicone
mouseWB,IF

Dis Model Mech

Loss of Ranbp2 in motor neurons causes the disruption of nucleocytoplasmic and chemokine signaling and proteostasis of hnRNPH3 and Mmp28, and the development of amyotrophic lateral sclerosis (ALS)-like syndromes.

Authors - Kyoung-In Cho
humanWB

Am J Pathol

CD133 affects the invasive ability of HCT116 cells by regulating TIMP-2.

Authors - Zhang Min M
humanWB

FASEB J

Drastic induction of MMP-7 by cortisol in the human amnion: implications for membrane rupture at parturition.

Authors - Lu-Yao Wang