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MTHFD1L Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 16113-1-AP

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Con: 38 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, ELISA
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Images See all

MTHFD1L Antibody WB HepG2 cells 16113-1-AP

HepG2 cells were subjected to SDS PAGE followed by western blot with 16113-1-AP(MTHFD1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

MTHFD1L Antibody IHC human colon cancer tissue 16113-1-AP

Immunohistochemical of paraffin-embedded human colon cancer using 16113-1-AP(MTHFD1L antibody) at dilution of 1:100 (under 10x lens)

MTHFD1L Antibody IHC human colon cancer tissue 16113-1-AP

Immunohistochemical of paraffin-embedded human colon cancer using 16113-1-AP(MTHFD1L antibody) at dilution of 1:100 (under 40x lens)

MTHFD1L Antibody IP HeLa cells 16113-1-AP

IP Result of anti-MTHFD1L (IP:16113-1-AP, 4ug; Detection:16113-1-AP 1:1000) with HeLa cells lysate 2000ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
HepG2 cells, COLO 320 cells, HeLa cells, mouse ovary tissue
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human colon cancer tissue
Recommended dilution:
WB : 1:500-1:3000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
MTHFD1L fusion protein Ag9036
Full name:
methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
Calculated molecular weight:
978aa,106 kDa
Observed molecular weight:
106 kDa
GenBank accession number:
BC017477
Gene ID (NCBI):
25902
Gene symbol
MTHFD1L
Synonyms
FTHFSDC1, MTC1THFS, MTHFD1L

Background

MTHFD1L(Monofunctional C1-tetrahydrofolate synthase, mitochondrial) is also named as FTHFSDC1(Formyltetrahydrofolate synthetase). MTHFD1L enzyme is present in mitochondria from normal embryonic tissues and embryonic fibroblast cell lines, and embryonic mitochondria possess the ability to synthesize formate from glycine. It catalyzes the final step in the mitochondrial conversion of 1-C units to formate in embryos. Moreover, MTHFD1L levels were substantially higher in embryonic mitochondria than in adult liver mitochondria and embryonic mitochondria exhibited greater formate production(PMID:19948730). It has 2 isoforms produced by alternative splicing.


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