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  • KD/KO Validated

MTX1 Polyclonal antibody

MTX1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 15529-1-AP

Synonyms

metaxin 1, MTX, MTX1, MTXN



Tested Applications

Positive WB detected inHepG2 cells, A549 cells, Jurkat cells, HeLa cells
Positive IP detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15529-1-AP targets MTX1 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTX1 fusion protein Ag7685
Full Name metaxin 1
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC001906
Gene Symbol MTX1
Gene ID (NCBI) 4580
RRIDAB_2148416
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

METAXINs are proteins located in the outer membrane of the mammalian mitochondrion. METAXIN 1 was characterized firstly and was found to play a role in the import of mitochondrial precursor proteins. It may also play a role in the assembly of b-barrel proteins into the outer membrane METAXIN1 was reported to be essential for survival of the postimplantation mouse embryo. METAXIN 1 has multiple transcripts. Several isoforms of METAXIN 1 exist due to the alternative splicing, with the predicted MW of 51, 48 and 36 kDa, respectively. Metaxin 1 interacts with metaxin 2, a novel related protein associated with the mammalian mitochondrial outer membrane and form complex in the mitochondrion outer membrane.

Protocols

Product Specific Protocols
WB protocol for MTX1 antibody 15529-1-APDownload protocol
IP protocol for MTX1 antibody 15529-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Int J Biol Sci

Genome-scale CRISPRa screening identifies MTX1 as a contributor for sorafenib resistance in hepatocellular carcinoma by augmenting autophagy.

Authors - Li Li
  • KD Validated
mouseWB

Hepatology

Loss of Sam50 in hepatocytes induces cardiolipin-dependent mitochondrial membrane remodeling to trigger mtDNA release and liver injury

Authors - Li Chen
humanWB

J Cell Sci

Myo19 tethers mitochondria to endoplasmic reticulum-associated actin to promote mitochondrial fission

Authors - Stephen M Coscia
  • KD Validated