MYC-tag Monoclonal antibody
MYC-tag Monoclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Recombinant protein and More (1)
Applications
WB, IP, IF, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
2D11A8
Cat no : 66004-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Recombinant protein |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:2000-1:20000 for WB |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 10 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
| CoIP | See 2 publications below |
Product Information
66004-1-Ig targets MYC-tag in WB, IP, IF, CoIP, ELISA applications and shows reactivity with Recombinant protein samples.
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYC-tag antibody 66004-1-Ig | Download protocol |
| IF protocol for MYC-tag antibody 66004-1-Ig | Download protocol |
| IP protocol for MYC-tag antibody 66004-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Ophthalmol Soluble expression of recomb inant cMyc, Klf4, Oct4, and Sox2 proteins in bacteria and transduction into living cells. | ||
Acta Biochim Biophys Sin (Shanghai) Protein kinase Cα inhibits myocardin-induced cardiomyocyte hypertrophy through the promotion of myocardin phosphorylation. | ||
Virol J Herpes simplex virus 1 UL41 protein abrogates the antiviral activity of hZAP by degrading its mRNA. | ||
Molecules Anti-HIV Activities and Mechanism of 12-O-Tricosanoylphorbol-20-acetate, a Novel Phorbol Ester from Ostodes katharinae. |
