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MYC-tag Antibody 32 Publications

Mouse Monoclonal| Catalog number: 66004-1-Ig |CloneNo.: 2D11A8

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Con: 100 μg/150 μl

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Tested Applications

Applications:
WB, IP, IF, CoIP, ELISA
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Images See all

MYC-tag Antibody WB Ag19172 66004-1-Ig

Western Blot of Myc-tagged fusion protein with mouse anti-Myc tag monoclonal antibody(66004-1-Ig) at various dilutions incubated at room temperature for 1.5 hours

MYC-tag Antibody IP Transfected HEK-293 cells 66004-1-Ig

IP Result of anti-MYC-tag (IP:66004-1-Ig, 3ug; Detection:66004-1-Ig 1:5000) with Transfected HEK-293 cells lysate 500ug.

MYC-tag Antibody IF Transfected HEK-293 cells 66004-1-Ig

Immunofluorescent analysis of Transfected HEK-293 cells using 66004-1-Ig(MYC-tag antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
Recombinant protein, human
Positive WB detected in:
Recombinant protein
Positive IP detected in:
Transfected HEK-293 cells
Positive IF detected in:
Transfected HEK-293 cells
Recommended dilution:
WB : 1:2000-1:20000
IP : 0.5-4.0 ug for IP and 1:2000-1:20000 for WB
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
MYC-tag
Calculated molecular weight:
Observed molecular weight:
GenBank accession number:
Gene ID (NCBI):
Gene symbol
Synonyms

Background

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.


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