"MYC tag Antibodies" Comparison
View side-by-side comparison of MYC tag antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | Transfected HEK-293T cells, recombinant proein |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
| Positive FC (Intra) detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60003-2-Ig targets MYC tag in WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, mouse, pig, yeast, recombinant protein |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | MYC tag |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2734122 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | MYCTAG |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for MYC tag antibody 60003-2-Ig | Download protocol |
| IF protocol for MYC tag antibody 60003-2-Ig | Download protocol |
| IP protocol for MYC tag antibody 60003-2-Ig | Download protocol |
| WB protocol for MYC tag antibody 60003-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MYC tag Monoclonal antibody (1A5A2), catalog number 60003-2-Ig, has 561 citations spanning journals including Nature, Nature Communications, Nature Cell Biology, Cell Research, Cancer Cell, Cell Death & Differentiation, Autophagy, Redox Biology, and Advanced Science. Research fields covered include cancer biology, ferroptosis, autophagy, immunology, metabolism, cell signaling, epigenetics, DNA damage repair, neurodegeneration, inflammation, and plant biology. The antibody is widely used in WB, IP, CoIP, IF, and ChIP applications across human, mouse, and recombinant protein studies.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Aberrant Notch-signaling promotes tumor angiogenesis in esophageal squamous-cell carcinoma | ||
Cancer Cell Inhibiting intracellular CD28 in cancer cells enhances antitumor immunity and overcomes anti-PD-1 resistance via targeting PD-L1 | ||
Mol Cancer ZDHHC20 mediated S-palmitoylation of fatty acid synthase (FASN) promotes hepatocarcinogenesis | ||
Cell Res Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P | ||
Cell Res Lysine 2-hydroxyisobutyrylation of NAT10 promotes cancer metastasis in an ac4C-dependent manner |
Reviews
What customers say
Most reviewers report strong performance for Western blot and immunoprecipitation, with clean bands at dilutions ranging from 1:500 to 1:10,000, particularly for detecting Myc-tagged proteins in HEK293 cells. Two reviewers noted excessive background, both using lot 10003656, and suggested alternative antibodies performed better. Overall, the antibody is well-received for its sensitivity and specificity in standard applications, though users should be mindful of lot-to-lot variability and optimize blocking conditions to minimize background.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
VASUDEVARAO (Verified Customer) (07-23-2025) | The antibody works very well at the recommended dilutions and performs excellently in both immunoprecipitation and immunoblotting applications.
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RASHMI (Verified Customer) (07-26-2024) | Used this product, highly recommended
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Sarah (Verified Customer) (10-27-2023) | works well at low dilution for WB and worked for immunoprecipitation
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manohar (Verified Customer) (07-10-2023) | Used Nitrocellulose membrane with 5% milk as blocking, antibody diluted in 1% milk.
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Amy (Verified Customer) (03-05-2023) | Clean bands for detecting over expressed Myc-tagged proteins in HEK293T cell lysates.
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Isabelle Cristine (Verified Customer) (01-14-2022) | The antibody works generally well for the detection of MYC-tagged proteins.
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Tom (Verified Customer) (02-10-2021) | HEK293T cells were transfected with a Myc-tagged construct. The 60003-2-Ig antibody (1:2000) resulted in a lot of background. An alternative antibody was used alongside this one, with a stronger band and less background being achieved.
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Alinda (Verified Customer) (10-09-2019) | This antibody was used at 1:1000 in HEK cells transfected with a myc-tagged insert cloned into an overxpression. Cells were blocked for 1 hr at room temp in 5%goat serum PBST.This antibody has a lot of background; another antibody from cell signalling was tested in parallel and performed much better
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