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MYD88 Polyclonal antibody

MYD88 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23230-1-AP

Synonyms

MYD88, MYD88D



Tested Applications

Positive WB detected inA549 cells, HepG2 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23230-1-AP is MYD88 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity African green monkey, human, sheep, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYD88 fusion protein Ag19770
Full Name myeloid differentiation primary response gene (88)
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC013589
Gene symbol MYD88
Gene ID (NCBI) 4615
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Myeloid differentiation primary response 88 (MYD88) is an adapter protein critical to the innate and adaptive immune response.

 What is the molecular weight of MYD88?

The molecular weight of MYD88 is 33 kDa.

 What is the cellular localization of MYD88?

The subcellular localization of MYD88 is largely confined to the cytoplasm as condensed forms or aggregated structures.

 What is the role of MYD88 in the IL-1R signaling pathway?

MYD88 plays a major role in the inflammatory signaling pathways downstream of Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) families. MYD88 links these receptors to IL-1R-associated kinases (IRAK), such as IRAK1 and IRAK2, via protein–protein interactions. The C-terminal TIR domain of MYD88 mediates the interaction with the receptors, whereas the N-terminal death domain of MYD88 associates with IRAK family members (PMID: 25580251). MYD88 acts via its intermediate domain to phosphorylate and thus activate IRAK1, IRAK2, IRF7, and TRAF6 to trigger NF-kappa-B signaling and cytokine secretion as part of the inflammatory response (PMID: 19679662).

 What is MYD88's involvement in disease?

Defects in MYD88 due to deficiency of the protein leads to recurrent pyogenic bacterial infections, including invasive pneumococcal disease. Patients usually die between 1 and 11 months of age, but surviving patients are otherwise healthy with normal resistance to other microbes (PMID: 18669862). Mutations in the MYD88 gene also lead to the development of cancers such as lymphoma (PMID: 21179087) and some autoimmune disorders like ulcerative colitis (PMID: 24189845).


Protocols

Product Specific Protocols
WB protocol for MYD88 antibody 23230-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Life Sci

Rosmarinic acid attenuates inflammatory responses through inhibiting HMGB1/TLR4/NF-κB signaling pathway in a mouse model of Parkinson's disease.

Authors - Runxiao Lv
ratWB

Molecules

Neuroprotective Effects of Ginsenosides against Cerebral Ischemia.

Authors - Zhekang Cheng
mouseWB

J Agric Food Chem

Comparison of the Hepatoprotective Effects of the Three Main Stilbenes from Mulberry Twigs.

Authors - Ya-Nan Jia
humanWB

Onco Targets Ther

Long noncoding RNA UBE2R2-AS1 promotes glioma cell apoptosis via targeting the miR-877-3p/TLR4 axis.

Authors - Wu Xu
ratWB

Biomed Pharmacother

Potential mechanisms underlying the protective effects of salvianic acid A against atherosclerosis in vivo and vitro.

Authors - Qiongtao Song
ratWB

Oxid Med Cell Longev

Methane-Rich Saline Counteracts Cholestasis-Induced Liver Damage via Regulating the TLR4/NF-κB/NLRP3 Inflammasome Pathway.

Authors - Zeyu Li