Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, K-562 cells, Neuro-2a cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse heart tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14611-1-AP targets MYO18A in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6185 Product name: Recombinant human MYO18A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1601-2002 aa of BC039612 Sequence: TLSDQVNRRDFESEKRLRKDLKRTKALLADAQLMLDHLKNSAPSKREIAQLKNQLEESEFTCAAAVKARKAMEVEIEDLHLQIDDIAKAKTALEEQLSRLQREKNEIQNRLEEDQEDMNELMKKHKAAVAQASRDLAQINDLQAQLEEANKEKQELQEKLQALQSQVEFLEQSMVDKSLVSRQEAKIRELETRLEFERTQVKRLESLASRLKENMEKLTEERDQRIAAENREKEQNKRLQRQLRDTKEEMGELARKEAEASRKKHELEMDLESLEAANQSLQADLKLAFKRIGDLQAAIEDEMESDENEDLINSEGDSDVDSELEDRVDGVKSWLSKNKGPSKAASDDGSLKSSSPTSYWKSLAPDRSDDEHDPLDNTSRPRYSHSYLSDSDTEAKLTETNA Predict reactive species |
| Full Name | myosin XVIIIA |
| Calculated Molecular Weight | 233 kDa |
| Observed Molecular Weight | 230 kDa, 190 kDa |
| GenBank Accession Number | BC039612 |
| Gene Symbol | MYO18A |
| Gene ID (NCBI) | 399687 |
| RRID | AB_2201447 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92614 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYO18A is an unconventional myosin that may play a role in the stabilization and organization of the actin cytoskeleton. Recently MYO18A has been identified as the receptor of SP and is responsible for clearance of SP-A-opsonized bacteria in in vivo infection. Two different isoforms of mouse Myo18A have been identified: Myo18A-alpha and Myo18A-beta as 230- and 190-kDa proteins, respectively. Myo18a-alpha colocalizes with the ER-Golgi complex and with membrane ruffles and filopodia, while Myo18a-beta localizes diffusely to the cytoplasm.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MYO18A antibody 14611-1-AP | Download protocol |
| IP protocol for MYO18A antibody 14611-1-AP | Download protocol |
| WB protocol for MYO18A antibody 14611-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
MYO18A Polyclonal antibody (Cat# 14611-1-AP) has 20 citations across journals including J Clin Invest, Cell Death Differ, Diabetes, Thorax, Cell Rep, Cell Syst, Sci Signal, Oncoimmunology, J Cell Sci, Sci Rep, Antiviral Res, J Biol Chem, Immunobiology, and Microbes Infect. Research fields span oncology (lung, pancreatic, cholangiocarcinoma, AML), cell biology (Golgi, myelination, hair cells), immunology (macrophages, NK cells, viral infection), diabetes, and pulmonary disease.
| Species | Application | Title |
|---|---|---|
J Clin Invest A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma.
| ||
J Clin Invest Proteogenomic analysis reveals cytoplasmic sequestration of RUNX1 by the acute myeloid leukemia-initiating CBFB::MYH11 oncofusion protein | ||
Cell Death Differ LncRNA BCAN-AS1 stabilizes c-Myc via N6-methyladenosine-mediated binding with SNIP1 to promote pancreatic cancer | ||
Cell Death Differ The Smad4-MYO18A-PP1A complex regulates β-catenin phosphorylation and pemigatinib resistance by inhibiting PAK1 in cholangiocarcinoma.
| ||
Diabetes Unbiased Profiling of the Human Proinsulin Biosynthetic Interaction Network Reveals a Role For Peroxiredoxin 4 in Proinsulin Folding. | ||
Thorax Advanced MicroRNA delivery for lung inflammatory therapy: surfactant protein A controls cellular internalisation and degradation of extracellular vesicles |
Reviews
What customers say
The single available review (rated 3/5) was posted in April 2024 for Western Blot on HUVEC cells at a 1:1000 dilution. The reviewer noted that the antibody detects several bands, with one appearing at the expected molecular weight. They recommended further validation via knockout or knockdown to confirm specificity. Overall, the feedback suggests the product works but may benefit from additional specificity verification.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sammy (Verified Customer) (04-22-2024) | Detects several bands. One at the correct MW that will need to be validated by KO/KD.
|
