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  • KD/KO Validated

Marcksl1 Polyclonal antibody

Marcksl1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10002-2-AP

Synonyms

AL022768, AW215397, AW536807, D4Bc1, F52, MacMARCKS, Macs 2, Macs 3, Macs2, Macs3, MARCKS like 1, Marcksl1, Mlp, Mrp



Tested Applications

Positive WB detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10002-2-AP targets Marcksl1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein
Full Name MARCKS-like 1
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC006757
Gene Symbol Marcksl1
Gene ID (NCBI) 17357
RRIDAB_513892
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MacMARCKS is a member of MARCKS family of PKC substrate. It is widely used in the signal transduction studies as an indictor of PKC activation. The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-45 kDa in different species on SDS PAGE. MacMARCKS functions in many aspects of cell physiology including integrin activation, cell spreading and phagocytosis. Its functions are though to associate with its ability in binding and regulating actin and microtubule cytoskeleton. Current evidence indicated that this protein also involved controls membrane fluidity and molecular mobility of membrane proteins

Protocols

Product Specific Protocols
WB protocol for Marcksl1 antibody 10002-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

J Exp Med

Development of intestinal M cells and follicle-associated epithelium is regulated by TRAF6-mediated NF-κB signaling.

Authors - Takashi Kanaya
mouseIF

Dev Cell

An integrated approach identifies the molecular underpinnings of murine anterior visceral endoderm migration

Authors - Shifaan Thowfeequ
humanWB,IHC

J Biol Chem

The long noncoding RNA lncZic2 drives the self-renewal of liver tumor-initiating cells via the protein kinase C substrates MARCKS and MARCKSL1.

Authors - Zhenzhen Chen
  • KO Validated
human,mouseWB,IHC

Mol Cell Biol

c-Jun N-terminal kinase phosphorylation of MARCKSL1 determines actin stability and migration in neurons and in cancer cells.

Authors - Bj?rkblom Benny B
  • KD Validated
mouseWB

PLoS One

Colonic miRNA Expression/Secretion, Regulated by Intestinal Epithelial PepT1, Plays an Important Role in Cell-to-Cell Communication during Colitis.

Authors - Saravanan Ayyadurai
humanWB,IF

Exp Cell Res

The regulation of MacMARCKS expression by integrin beta3.

Authors - van den Bout Iman I