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- KD/KO Validated
GRIM19 Polyclonal antibody
GRIM19 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 10986-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, HEK-293 cells, MCF-7 cells, rat heart tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human prostate cancer tissue, human heart tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IHC | See 4 publications below |
Product Information
10986-1-AP targets GRIM19 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GRIM19 fusion protein Ag1435 |
| Full Name | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13 |
| Calculated Molecular Weight | 16.5 kDa |
| Observed Molecular Weight | 16-18 kDa |
| GenBank Accession Number | BC009189 |
| Gene Symbol | NDUFA13 |
| Gene ID (NCBI) | 51079 |
| RRID | AB_2150609 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GRIM19(Gene associated with retinoic and interferon-induced mortality 19 protein) is also named as NDUFA13 ,CI-B16.6 and belongs to the complex I NDUFA13 subunit family.t GRIM-19 may distribute in the mitochondria and/or nucleus depending on the cell type.Alternatively, its cellular localization may be regulated by post-translational modifications such as phosphorylation and acetylation. Lastly, GRIM-19 may exist in different isoforms that may be distributed in distinct cellular locations(PMID:12628925).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GRIM19 antibody 10986-1-AP | Download protocol |
| IHC protocol for GRIM19 antibody 10986-1-AP | Download protocol |
| IP protocol for GRIM19 antibody 10986-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Dev Cell PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine. | ||
Hum Gene Ther Nonviral delivery of GRIM-19 gene inhibits tumor growth with reduced local and systemic complications. | ||
Exp Cell Res GRIM-19 inhibits proliferation and induces apoptosis in a p53-dependent manner in colorectal cancer cells through the SIRT7/PCAF/MDM2 axis.
| ||
Int J Oncol Atrazine promotes RM1 prostate cancer cell proliferation by activating STAT3 signaling. | ||
Oncol Lett GRIM-19 deficiency promotes clear cell renal cell carcinoma progression and is associated with high TNM stage and Fuhrman grade. |
