NDUFS1 Antibody 13 Publications

Rabbit Polyclonal| Catalog number: 12444-1-AP

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Freight/Packing

Con: 25 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
human kidney tissue, A549 cells, human kidney tissue

Positive IP detected in:
mouse lung tissue

Positive IHC detected in:
human gliomas tissue

Positive IF detected in:
Hela cells

Recommended dilution:
WB : 1:200-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase)

Calculated molecular weight:
727aa,79 kDa

Observed molecular weight:
79 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
NDUFS1

Synonyms
CI 75Kd, Complex I 75kD, NDUFS1, PRO1304
Background

The multisubunit NADH:ubiquinone oxidoreductase (75 kDa subunit, mitochondrial)(NDUFS1) is the first enzyme complex in the electron transport chain of mitochondria. It is also named as Complex I-75kD. By use of chaotropic agents, complex I can be fragmented into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. NDUFS1 is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme.


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