NEDD8 Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 16777-1-AP

  • Print page
  • Download PDF

-_-

Freight/Packing

Con: 45 μg/150 μl

Choose size:

Please visit your regions distributor:


Species specificity:
human, mouse, rat

Positive WB detected in:
mouse brain tissue, human heart tissue, mouse brain tissue

Positive IHC detected in:
human colon cancer tissue

Recommended dilution:
WB : 1:500-1:2000
IHC : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
neural precursor cell expressed, developmentally down-regulated 8

Calculated molecular weight:
81aa,9 kDa

Observed molecular weight:
6-10 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
NEDD8

Synonyms
cul nedd8, FLJ43224, Nedd 8, NEDD8, Neddylin, Ubiquitin like protein Nedd8
Background

NEDD8 (neural precursor cell-expressed developmentally down-regulated gene 8) is a small ubiquitin-like protein that shares 60% sequence identity and 80% homology with ubiquitin [PMID:9353319]. NEDD8 is conjugated to substrate proteins in a process known as neddylation. NEDD8 forms a thioester bond with APPBP1-Uba3, the E1 enzyme. Activated NEDD8 then transfers to Ubc12, the E2 enzyme . Ultimately, E2 loaded with NEDD8 binds a substrate protein lysine residue and forms an isopeptide bond by E3 ligase [PMID:18802447].


Back
to top