NF-H Polyclonal antibody

NF-H Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18934-1-AP

Synonyms

NEFH, NEH, NF, NF H, NF200



Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18934-1-AP is NF-H Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NF-H fusion protein Ag13517
Full Name neurofilament, heavy polypeptide
Calculated molecular weight 112 kDa
Observed molecular weight 200 kDa, 140-160 kDa
GenBank accession numberBC014185
Gene symbol NEFH
Gene ID (NCBI) 4744
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NEFH (NF200), also named as KIAA0845 and NFH, belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody can recognize both NEFH and NEFM.

Protocols

Product Specific Protocols
WB protocol for NF-H antibody 18934-1-APDownload protocol
IHC protocol for NF-H antibody 18934-1-APDownload protocol
IF protocol for NF-H antibody 18934-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Acta Neuropathol Commun

A robust TDP-43 knock-in mouse model of ALS.

Authors - Shih-Ling Huang
ratWB

Neural Regen Res

Bone marrow mesenchymal stem cells repair spinal cord ischemia/reperfusion injury by promoting axonal growth and anti-autophagy.

Authors - Fei Yin
ratIHC

Neural Regen Res

Endothelial progenitor cell-conditioned medium promotes angiogenesis and is neuroprotective after spinal cord injury.

Authors - Tao Wang
ratWB

Cell Biochem Funct

The mechanisms of EGFR in the regulation of axon regeneration.

Authors - Xu Ming-Feng MF
ratIF

Evid Based Complement Alternat Med

Effects of Ginkgo biloba on Early Decompression after Spinal Cord Injury.

Authors - Xing Guo
mouseIHC

J Neuroimmunol

Methylene blue alleviates experimental autoimmune encephalomyelitis by modulating AMPK/SIRT1 signaling pathway and Th17/Treg immune response.

Authors - Jueqiong Wang