NF-H Polyclonal antibody

NF-H Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 18934-1-AP



Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18934-1-AP is NF-H Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NF-H fusion protein Ag13517
Full Name neurofilament, heavy polypeptide
Calculated molecular weight 112 kDa
Observed molecular weight 200 kDa, 140-160 kDa
GenBank accession numberBC014185
Gene symbol NEFH
Gene ID (NCBI) 4744
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NEFH (NF200), also named as KIAA0845 and NFH, belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody can recognize both NEFH and NEFM.


Product Specific Protocols
WB protocol for NF-H antibody 18934-1-APDownload protocol
IHC protocol for NF-H antibody 18934-1-APDownload protocol
IF protocol for NF-H antibody 18934-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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