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NF-H Antibody 8 Publications

Rabbit Polyclonal| Catalog number: 18934-1-AP

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Con: 49 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

NF-H Antibody IHC mouse brain tissue 18934-1-AP

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18934-1-AP (NF-H antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

NF-H Antibody WB mouse brain tissue 18934-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 18934-1-AP(NEFH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

NF-H Antibody WB mouse brain tissue 18934-1-AP

WB result of 18934-1-AP.

NF-H Antibody IHC mouse brain tissue 18934-1-AP

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18934-1-AP (NF-H antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

NF-H Antibody IF mouse brain tissue 18934-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed mouse brain tissue using 18934-1-AP (NF-H antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse brain tissue
Positive IHC detected in:
mouse brain tissue, human gliomas tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
mouse brain tissue
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
NF-H fusion protein Ag13517
Full name:
neurofilament, heavy polypeptide
Calculated molecular weight:
112 kDa
Observed molecular weight:
200 kDa,140-160 kDa
GenBank accession number:
BC014185
Gene ID (NCBI):
4744
Gene symbol
NEFH
Synonyms
NEFH, NEH, NF H, NF200

Background

NEFH(NF200), also named as KIAA0845 and NFH, Belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE:NF-L, 65-68kd; NF-M,145-160kd and NF-H, 200-220kd. This antibody can recognize both NEFH and NEFM.


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