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NIP7 Polyclonal antibody

NIP7 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 16839-1-AP

Synonyms

CGI 37, HSPC031, HSPC180, KD93, NIP7, OK/SW cl.76, OK/SW cl.78

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NIP7 Polyclonal antibody (16839-1-AP)

    WB analysis using 16839-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16839-1-AP (NIP7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using NIP7 Polyclonal antibody (16839-1-AP)

    IP experiment of HeLa using 16839-1-AP

    IP result of anti-NIP7 (IP:16839-1-AP, 4ug; Detection:16839-1-AP 1:1000) with HeLa cells lysate 1600 ug.

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16839-1-AP targets NIP7 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NIP7 fusion protein Ag10526
Full Name nuclear import 7 homolog (S. cerevisiae)
Calculated Molecular Weight 180 aa, 20 kDa
Observed Molecular Weight 20-22 kDa
GenBank Accession NumberBC015941
Gene Symbol NIP7
Gene ID (NCBI) 51388
RRIDAB_2153549
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nip7 was initially identified in yeast as required for processing of the 27S pre-rRNA to form the mature 25S and 5.8S rRNAs (PMID: 9891085). It localizes to the nucleolus but was also found to sediment in the region of free 60S subunits in sucrose density gradients (PMID: 9891085). Experimental evidence suggests that the P. abyssi Nip7 may be an exosome regulatory factor. It binds preferentially to U- and AU-rich RNAs and strongly inhibits the exosome due to its association with both the exosome complex and the substrate RNA.

Protocols

Product Specific Protocols
WB protocol for NIP7 antibody 16839-1-APDownload protocol
IP protocol for NIP7 antibody 16839-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols