NMD3 Polyclonal antibody

NMD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16060-1-AP

Synonyms

CGI 07, hNMD3, NMD3



Tested Applications

Positive WB detected inHepG2 cells, L02 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman breast cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16060-1-AP targets NMD3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NMD3 fusion protein Ag8894
Full Name NMD3 homolog (S. cerevisiae)
Calculated Molecular Weight 503 aa, 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC013317
Gene Symbol NMD3
Gene ID (NCBI) 51068
RRIDAB_2282830
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human NMD3 gene encodes 60S ribosomal export protein which was found in a 60S ribosomal subunit export complex with RAN and XPO1. This nucleocytoplasmic shuttling protein NMD3 is an adaptor for export of the 60S ribosomal subunit from the nucleus. NMD3 contains a CRM-1-dependent leucine-rich nuclear export signal (NES) and a dispersed nuclear localization signal (NLS), the basic region of which is also required for nucleolar accumulation. NMD3 are required for nuclear export of the 60S ribosomal subunit in yeast and vertebrate cells, recent finding has also revealed its role in Arabidopsis thaliana.

Protocols

Product Specific Protocols
WB protocol for NMD3 antibody 16060-1-APDownload protocol
IHC protocol for NMD3 antibody 16060-1-APDownload protocol
IF protocol for NMD3 antibody 16060-1-APDownload protocol
IP protocol for NMD3 antibody 16060-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Stem Cell

HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis.

Authors - Kaosheng Lv
mouseWB

Genes Dev

Uncoupling of GTP hydrolysis from eIF6 release on the ribosome causes Shwachman-Diamond syndrome.

Authors - Andrew J Finch
humanWB

Blood

Defective ribosome assembly in Shwachman-Diamond syndrome.

Authors - Wong Chi C CC
humanIF

Nucleic Acids Res

The induction of p53 correlates with defects in the production, but not the levels, of the small ribosomal subunit and stalled large ribosomal subunit biogenesis

Authors - Matthew John Eastham
humanWB

Cancer Lett

RNA-binding protein hnRNPU regulates multiple myeloma resistance to selinexor

Authors - Xin Wang
human,mouseWB

Nature

Synthesis of portimines reveals the basis of their anti-cancer activity

Authors - Junchen Tang
  • KD Validated