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  • Featured Product
  • KD/KO Validated

NMT1 Polyclonal antibody

NMT1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11546-1-AP

Synonyms

N myristoyltransferase 1, NMT1, Type I N myristoyltransferase

Validation Data Gallery

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  • Western Blot (WB) analysis of SKOV-3 cells using NMT1 Polyclonal antibody (11546-1-AP)

    WB analysis of SKOV-3 using 11546-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NMT1 Polyclonal antibody (11546-1-AP)

    WB analysis of HeLa using 11546-1-AP

    WB result of NMT1 antibody (11546-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NMT1 transfected HeLa cells.

  • Western Blot (WB) analysis of mouse pancreas tissue using NMT1 Polyclonal antibody (11546-1-AP)

    WB analysis of mouse pancreas using 11546-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using NMT1 Polyclonal antibody (11546-1-AP)

    WB analysis of human kidney using 11546-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NMT1 Polyclonal antibody (11546-1-AP)

    WB analysis of HeLa using 11546-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using NMT1 Polyclonal antibody (11546-1-AP)

    WB analysis of L02 using 11546-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using NMT1 Polyclonal antibody (11546-1-AP)

    WB analysis of PC-3 using 11546-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using NMT1 Polyclonal antibody (11546-1-AP)

    IP experiment of HeLa using 11546-1-AP

    IP result of anti-NMT1 (IP:11546-1-AP, 4ug; Detection:11546-1-AP 1:500) with HeLa cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using NMT1 Polyclonal antibody (11546-1-AP)

    IHC staining of human gliomas using 11546-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11546-1-AP (NMT1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using NMT1 Polyclonal antibody (11546-1-AP)

    IHC staining of human heart using 11546-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 11546-1-AP (NMT1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NMT1 Polyclonal antibody (11546-1-AP)

    IHC staining of human kidney using 11546-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11546-1-AP (NMT1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NMT1 Polyclonal antibody (11546-1-AP)

    IHC staining of human kidney using 11546-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11546-1-AP (NMT1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using NMT1 Polyclonal antibody (11546-1-AP)

    IHC staining of human heart using 11546-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 11546-1-AP (NMT1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using NMT1 Polyclonal antibody (11546-1-AP)

    IF Staining of MCF-7 using 11546-1-AP

    Immunofluorescent analysis of MCF-7 cells, using NMT1 antibody 11546-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inSKOV-3 cells, PC-3 cells, mouse pancreas tissue, HeLa cells, L02 cells, human kidney tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue, human heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11546-1-AP targets NMT1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NMT1 fusion protein Ag2072
Full Name N-myristoyltransferase 1
Calculated Molecular Weight 496 aa, 57 kDa
Observed Molecular Weight 49-68 kDa
GenBank Accession NumberBC006569
Gene Symbol NMT1
Gene ID (NCBI) 4836
RRIDAB_2153157
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NMT1 is a N-myristoyltransferase responsible for the transfer of myristate from CoA to an amino-terminal glycine of many eukaryotic proteins, which facilitates the targeting of proteins to membrane surfaces and is essential for viability of the organism. Insertional mutagenesis of the Nmt1 gene in Saccharomyces cerevisiae causes recessive lethality. Humans and mice possess two distinct but structurally similar enzymes, NMT1 and NMT2, ubiquitously expressed in most human and mouse tissues. Western analysis revealed that there are 4 isoforms of NMT1 with apparent molecular masses ranging from 49 to 68 kDa. In cell fractionation studies, the 68-kDa NMT1 isoform and NMT2 were present in both membrane and cytoplasmic fractions, while the smaller NMT1 isoforms were predominantly cytoplasmic.

Protocols

Product Specific Protocols
WB protocol for NMT1 antibody 11546-1-APDownload protocol
IHC protocol for NMT1 antibody 11546-1-APDownload protocol
IF protocol for NMT1 antibody 11546-1-APDownload protocol
IP protocol for NMT1 antibody 11546-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Brain

Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA.

Authors - Janja Božič
View Article
humanWB,IF

Cancer Lett

N-myristoyltransferase-1 deficiency blocks myristoylation of LAMTOR1 and inhibits bladder cancer progression.

Authors - Yi Sun
  • KD Validated
View Article
humanWB,IHC

Cell Death Dis

NMT1 inhibition modulates breast cancer progression through stress-triggered JNK pathway.

Authors - Lu Deng
  • KD Validated
View Article
humanWB

FASEB J

Regulation of co- and post-translational myristoylation of proteins during apoptosis: interplay of N-myristoyltransferases and caspases.

Authors - Perinpanayagam Maneka A MA
View Article
mouseWB

Biochem Biophys Res Commun

N-myristoyltransferase 1 interacts with calnexin at the endoplasmic reticulum.

Authors - Elzbieta Dudek
View Article
human

Oncotarget

Tris DBA palladium is highly effective against growth and metastasis of pancreatic cancer in an orthotopic model.

Authors - Begoña Díaz
View Article