NMT1 Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 11546-1-AP

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Freight/Packing

Con: 45 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
SKOV-3 cells, HeLa cells, human kidney tissue, L02 cells, mouse pancreas tissue, PC-3 cells

Positive IP detected in:
HeLa cells

Positive IHC detected in:
human gliomas tissue, human heart tissue, human kidney tissue

Positive IF detected in:
MCF-7 cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
N-myristoyltransferase 1

Calculated molecular weight:
496aa,57 kDa

Observed molecular weight:
49-68 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
NMT1

Synonyms
N myristoyltransferase 1, NMT1, Type I N myristoyltransferase
Background

NMT1 is a N-myristoyltransferase responsible for the transfer of myristate from CoA to an amino-terminal glycine of many eukaryotic proteins, which facilitates the targeting of proteins to membrane surfaces and is essential for viability of the organism. Insertional mutagenesis of the Nmt1 gene in Saccharomyces cerevisiae causes recessive lethality. Humans and mice possess two distinct but structurally similar enzymes, NMT1 and NMT2, ubiquitously expressed in most human and mouse tissues. Western analysis revealed that there are 4 isoforms of NMT1 with apparent molecular masses ranging from 49 to 68 kD. In cell fractionation studies, the 68-kD NMT1 isoform and NMT2 were present in both membrane and cytoplasmic fractions, while the smaller NMT1 isoforms were predominantly cytoplasmic.


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