|Positive WB detected in||human adrenal gland tissue, human heart tissue, human liver tissue|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13442-2-AP targets NNT in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NNT fusion protein Ag4259|
|Full Name||nicotinamide nucleotide transhydrogenase|
|Calculated molecular weight||1085 aa, 114 kDa|
|Observed molecular weight||114 kDa|
|GenBank accession number||BC032370|
|Gene ID (NCBI)||23530|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
NNT(nicotinamide nucleotide transhydrogenase) is a transmembrane protein and functions as a proton pumping transhydrogenase. The protein is present in both prokaryotes and eukaryotes and is located in the inner membrane of mitochondria. In prokaryotic cells, the enzyme is composed of α and βsubunits of 54 and 48 kDa, respectively. In eukaryotic cells, the enzyme is usually composed of a single peptide of 110 kDa. Although NNT catalyzes the interconversion of NADH and NADPH, the forward reaction using the reducing power of NADH to regenerate NADPH would be favored under conditions of oxidative stress(PMID:16497723). It can exsit as a homodimer(PMID:21882037).
|Product Specific Protocols|
|WB protocol for NNT antibody 13442-2-AP||Download protocol|
|IHC protocol for NNT antibody 13442-2-AP||Download protocol|
|IF protocol for NNT antibody 13442-2-AP||Download protocol|
|IP protocol for NNT antibody 13442-2-AP||Download protocol|
|Click here to view our Standard Protocols|
Impact of a novel homozygous mutation in nicotinamide nucleotide transhydrogenase on mitochondrial DNA integrity in a case of familial glucocorticoid deficiency.
Int J Clin Exp Pathol
Nicotinamide nucleotide transhydrogenase acts as a new prognosis biomarker in hepatocellular carcinoma.
Mol Nutr Food Res
Lack of β, β-carotene -9', 10'-oxygenase 2 leads to hepatic mitochondrial dysfunction and cellular oxidative stress in mice.
Cell Physiol Biochem
Effect of Type I Diabetes on the Proteome of Mouse Oocytes.
Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice.
Nicotinamide nucleotide transhydrogenase-mediated redox homeostasis promotes tumor growth and metastasis in gastric cancer.