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NNT Antibody 5 Publications

Rabbit Polyclonal| Catalog number: 13442-2-AP

Featured Product KD/KO validated

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Con: 23 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

NNT Antibody WB human adrenal gland tissue 13442-2-AP

human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 13442-2-AP(NNT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

NNT Antibody IHC human liver cancer tissue 13442-2-AP

Immunohistochemical of paraffin-embedded human liver cancer using 13442-2-AP(NNT antibody) at dilution of 1:50 (under 10x lens)

NNT Antibody IHC human liver cancer tissue 13442-2-AP

Immunohistochemical of paraffin-embedded human liver cancer using 13442-2-AP(NNT antibody) at dilution of 1:50 (under 40x lens)

NNT Antibody IP HepG2 cells 13442-2-AP

IP Result of anti-NNT (IP:13442-2-AP, 4ug; Detection:13442-2-AP 1:300) with HepG2 cells lysate 2800ug.

NNT Antibody IF HeLa cells 13442-2-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 13442-2-AP(NNT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
human adrenal gland tissue, human heart tissue, human liver tissue
Positive IP detected in:
HepG2 cells
Positive IHC detected in:
human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
NNT fusion protein Ag4259
Full name:
nicotinamide nucleotide transhydrogenase
Calculated molecular weight:
1085aa,114 kDa
Observed molecular weight:
114 kDa
GenBank accession number:
BC032370
Gene ID (NCBI):
23530
Gene symbol
NNT
Synonyms
NNT

Background

NNT(nicotinamide nucleotide transhydrogenase) is a transmembrane protein and functions as a proton pumping transhydrogenase. The protein is present in both prokaryotes and eukaryotes and is located in the inner membrane of mitochondria. In prokaryotic cells, the enzyme is composed of α and βsubunits of 54 and 48 kDa, respectively. In eukaryotic cells, the enzyme is usually composed of a single peptide of 110 kDa. Although NNT catalyzes the interconversion of NADH and NADPH, the forward reaction using the reducing power of NADH to regenerate NADPH would be favored under conditions of oxidative stress(PMID:16497723). It can exsit as a homodimer(PMID:21882037).


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