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B23/NPM1 Monoclonal antibody

B23/NPM1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4F12A3

Cat no : 60096-1-Ig

Synonyms

B23, NPM, NPM1, Nucleophosmin, Numatrin



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, C6 cells, ROS1728 cells, NIH/3T3 cells, RAW 264.7 cells, MCF-7 cells, HEK-293 cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:200
Sample-dependent, check data in validation data gallery

Product Information

60096-1-Ig targets B23/NPM1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen B23/NPM1 fusion protein Ag7415
Full Name nucleophosmin (nucleolar phosphoprotein B23, numatrin)
Calculated molecular weight 33 kDa
Observed molecular weight 35-38 kDa
GenBank accession numberBC002398
Gene symbol NPM1
Gene ID (NCBI) 4869
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.

Protocols

Product Specific Protocols
WB protocol for B23/NPM1 antibody 60096-1-IgDownload protocol
IHC protocol for B23/NPM1 antibody 60096-1-IgDownload protocol
IF protocol for B23/NPM1 antibody 60096-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Heliyon

Tight expression regulation of senataxin, linked to motor neuron disease and ataxia, is required to avert cell-cycle block and nucleolus disassembly.

Authors - Craig L Bennett
humanWB

Nat Metab

Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.

Authors - Shi-Ting Li
humanIHC

Oncol Lett

Identification of the key genes and pathways in prostate cancer.

Authors - Shutong Fan
humanWB

Tumour Biol

Depletion of Dicer promotes epithelial ovarian cancer progression by elevating PDIA3 expression.

Authors - Ying Zhu
humanIF

PLoS One

HP1-mediated formation of alternative lengthening of telomeres-associated PML bodies requires HIRA but not ASF1a.

Authors - Jiang Wei-Qin WQ
humanIF

J Mol Biol

Proteomic characterization of the nucleolar linker histone H1 interaction network.

Authors - Heather J Szerlong