|Positive WB detected in||HEK-293 cells, A431 cells, C6 cells, Jurkat cells, NIH/3T3 cells|
|Positive IHC detected in||human pancreas tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10724-1-AP targets NRAS in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NRAS fusion protein Ag1081|
|Full Name||neuroblastoma RAS viral (v-ras) oncogene homolog|
|Calculated molecular weight||21 kDa|
|Observed molecular weight||21 kDa|
|GenBank accession number||BC005219|
|Gene ID (NCBI)||4893|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
NRAS, also named as N-ras and NRAS1, is neuroblastoma RAS viral (v-ras) oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML).
|Product Specific Protocols|
|WB protocol for NRAS antibody 10724-1-AP||Download protocol|
|IHC protocol for NRAS antibody 10724-1-AP||Download protocol|
|IF protocol for NRAS antibody 10724-1-AP||Download protocol|
|FC protocol for NRAS antibody 10724-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Brandon (Verified Customer) (11-27-2018)
Worked excellent on mouse tissue from inducible tumor model!