Tested Applications
| Positive WB detected in | HT-1080 cells, HeLa cells, HepG2 cells, SU-DHL-4 cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells, HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:750-1:3000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20854-1-AP targets NSUN2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14791 Product name: Recombinant human NSUN2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 415-767 aa of BC001041 Sequence: HHQNTGGFFVAVLVKKSSMPWNKRQPKLQGKSAETRESTQLSPADLTEGKPTDPSKLESPSFTGTGDTEIAHATEDLENNGSKKDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQLYMVSKELRNVLLNNSEKMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFFRKLSSETYSQAKDLAKGSIVLKYEPDSANPDALQCPIVLCGWRGKASIRTFVPKNERLHYLRMMGLEVLGEKKKEGVILTNESAASTGQPDNDVTEGQRAGEPNSPDAEEANSPDVTAGCDPAGVHPPR Predict reactive species |
| Full Name | NOL1/NOP2/Sun domain family, member 2 |
| Calculated Molecular Weight | 767 aa, 86 kDa |
| Observed Molecular Weight | 90-100 kDa |
| GenBank Accession Number | BC001041 |
| Gene Symbol | NSUN2 |
| Gene ID (NCBI) | 54888 |
| RRID | AB_10693629 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q08J23 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for NSUN2 antibody 20854-1-AP | Download protocol |
| IF protocol for NSUN2 antibody 20854-1-AP | Download protocol |
| IHC protocol for NSUN2 antibody 20854-1-AP | Download protocol |
| IP protocol for NSUN2 antibody 20854-1-AP | Download protocol |
| WB protocol for NSUN2 antibody 20854-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
20854-1-AP (anti-NSUN2 antibody) has accumulated 130 citations published in high-impact journals including Cell, Nature Communications, Nature Biotechnology, Molecular Cell, Cell Research, and Nucleic Acids Research. Research fields span RNA epigenetics and m5C modification, cancer biology (lung, breast, gastric, colorectal, ovarian), ferroptosis, virology (HIV, HBV, SARS-CoV-2), neuroscience, immunology, metabolism, and developmental biology.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther tRF-Gln-CTG-026 ameliorates liver injury by alleviating global protein synthesis | ||
Cell The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity. | ||
Nat Biotechnol Identification of direct targets and modified bases of RNA cytosine methyltransferases. | ||
Mol Cancer NSUN2-mediated cytosine-5 methylation of FSP1 protects acute myeloid leukemia cells from ferroptosis.
| ||
Cell Metab NSUN2 is a glucose sensor suppressing cGAS/STING to maintain tumorigenesis and immunotherapy resistance | ||
Cell Res 5-methylcytosine promotes mRNA export - NSUN2 as the methyltransferase and ALYREF as an m(5)C reader.
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Reviews
What customers say
Both reviewers gave 5-star ratings for Western Blot applications. One user confirmed the antibody performed well in lysates of murine CD4 T cells activated with aCD3/aCD28 at a 1:10,000 dilution. The other reported it worked perfectly on HeLa and HEK293T cell lysates at a 1:1,000 dilution. Overall, users find this antibody reliable and effective for WB across different cell types and species.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mirna (Verified Customer) (10-02-2025) | The antibody worked well in the lysate of murine CD4 T cells activated with aCD3aCD28.
 |
Sarah (Verified Customer) (01-05-2021) | Works perfectly for WB. Tested on HeLa cells and HEK293T.
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