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  • KD/KO Validated

NUDT6 Polyclonal antibody

NUDT6 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11181-1-AP

Synonyms

ASFGF2, FGF 2, FGF AS, FGF2AS, gfg, gfg 1, Nudix motif 6, NUDT6, Protein GFG



Tested Applications

Positive WB detected inJurkat cells, HepG2 cells, human heart tissue, rat heart tissue, rat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11181-1-AP targets NUDT6 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUDT6 fusion protein Ag1638
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 6
Calculated molecular weight 36 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC009842
Gene symbol NUDT6
Gene ID (NCBI) 11162
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NUDT6, also named as FGF2AS and GFG, belongs to the Nudix hydrolase family. It may contribute to the regulation of cell proliferation. NUDT6 maybe a candidate gene for fat deposition. The 72 kDa is a homodimer form of NUDT6.

Protocols

Product Specific Protocols
WB protocol for NUDT6 antibody 11181-1-APDownload protocol
IHC protocol for NUDT6 antibody 11181-1-APDownload protocol
IF protocol for NUDT6 antibody 11181-1-APDownload protocol
IP protocol for NUDT6 antibody 11181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Burns

Study of the use of recombinant human granulocyte-macrophage colony-stimulating factor hydrogel externally to treat residual wounds of extensive deep partial-thickness burn.

Authors - Lin Yuan
humanWB

Wound Repair Regen

GHK-Cu-liposomes accelerate scald wound healing in mice by promoting cell proliferation and angiogenesis.

Authors - Xinying Wang
ratWB

Genes (Basel)

Regulation of fibroblast growth factor-2 expression and cell cycle progression by an endogenous antisense RNA.

Authors - Mark Baguma-Nibasheka
  • KD Validated
mouseWB

Cancer Sci

PMID: 29532562

Authors - Ji-Chang Wang
humanWB

Mol Carcinog

Regulation of FGF-2 by an endogenous antisense RNA: effects on cell adhesion and cell-cycle progression.

Authors - MacFarlane Leigh-Ann LA
humanWB

Mol Endocrinol

Regulation of fibroblast growth factor-2 by an endogenous antisense RNA and by argonaute-2.

Authors - MacFarlane Leigh-Ann LA