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TXNL6 Polyclonal antibody

TXNL6 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11203-1-AP

Synonyms

nucleoredoxin like 1, Nucleoredoxin like protein 1, NXNL1, RDCVF, Thioredoxin like protein 6, TXNL6

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using TXNL6 Polyclonal antibody (11203-1-AP)

    WB analysis of mouse brain using 11203-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11203-1-AP (TXNL6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TXNL6 Polyclonal antibody (11203-1-AP)

    WB analysis of HeLa using 11203-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11203-1-AP (TXNL6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using TXNL6 Polyclonal antibody (11203-1-AP)

    IP experiment of mouse brain using 11203-1-AP

    IP result of anti-TXNL6 (IP:11203-1-AP, 4ug; Detection:11203-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TXNL6 Polyclonal antibody (11203-1-AP)

    IHC staining of human breast cancer using 11203-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11203-1-AP (TXNL6 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TXNL6 Polyclonal antibody (11203-1-AP)

    IHC staining of human breast cancer using 11203-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 11203-1-AP (TXNL6 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TXNL6 Polyclonal antibody (11203-1-AP)

    IHC staining of human breast cancer using 11203-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 11203-1-AP (TXNL6 Antibody) at dilution of 1:50.

Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11203-1-AP targets TXNL6 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TXNL6 fusion protein Ag1691
Full Name nucleoredoxin-like 1
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 26-28 kDa
GenBank Accession NumberBC014127
Gene Symbol NXNL1
Gene ID (NCBI) 115861
RRIDAB_2158126
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TXNL6, also named as NXNL1, belongs to the nucleoredoxin family. It may play a role in cone cell viability, slowing down cone degeneration, does not seem to play a role in degenerating rods. TXNL6 is a Novel Oxidative Stress-Induced Reducing System for Methionine Sulfoxide Reductase A Repair of a-Crystallin and Cytochrome C in the Eye Lens. It serve as a reducing system for MsrA repair of the essential lens chaperone a-crystallin/sHSP and mitochondrial cytochrome c. TXNL6 is induced at high levels in human lens epithelial cells exposed to H2O2-induced oxidative stress.(PMID:21079812)

Protocols

Product Specific Protocols
WB protocol for TXNL6 antibody 11203-1-APDownload protocol
IHC protocol for TXNL6 antibody 11203-1-APDownload protocol
IP protocol for TXNL6 antibody 11203-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigIF

Cell Rep

Two-Step Reactivation of Dormant Cones in Retinitis Pigmentosa.

Authors - Wei Wang
View Article
humanWB,IF

PLoS One

TXNL6 is a novel oxidative stress-induced reducing system for methionine sulfoxide reductase a repair of α-crystallin and cytochrome C in the eye lens.

Authors - Brennan Lisa A LA
View Article