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OCT4 Polyclonal antibody

OCT4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 11263-1-AP

Synonyms

Oct 3, Oct 4, OCT3, OCT3/4, OCT4, Octamer binding protein 3, Octamer binding protein 4, OTF 3, OTF3, OTF4, POU class 5 homeobox 1, POU5F1



Tested Applications

Positive WB detected inH7ES cells, HEK-293 cells, hESC cells, human placenta tissue, mouse brain tissue, mouse embryo tissue, mouse kidney tissue, NCCIT cell

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

11263-1-AP targets OCT4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OCT4 fusion protein Ag1794
Full Name POU class 5 homeobox 1
Calculated molecular weight 39 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC020712
Gene symbol POU5F1
Gene ID (NCBI) 5460
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Background

Octamer-binding protein 4 (OCT4), also known as POU5F1 or OCT3/4, is a member of the POU gene family and encoded by the human gene POU5F1 (POU domain class 5 transcription factor 1). OCT4 is a member of the Octamer class of transcription factors that recognize the 8-base pair consensus motif 5’-ATGCAAAT-3’. Expression of OCT4 is associated with an undifferentiated, pluripotent stem cell phenotype and tumors. OCT4 is also expressed in the developing brain, with the highest levels found in the cortex, olfactory bulb, and the hippocampus.

 What is the molecular weight of OCT4?

The molecular weight of OCT4 is 38 kDa.

 What are the isoforms of OCT4?

The human POU51F gene consists of five exons located on chromosome 6 and can generate three mRNA isoforms through alternative splicing – OCT4A, OCT4B, and OCT4B1 (PMID: 18787205). OCT4A and OCT4B1 orchestrate gene transcription in the nucleus supporting self-renewal and pluripotency maintenance in ESCs and embryonal carcinoma cells, whereas OCT4B is localized in the cytoplasm in various non-pluripotent cell types and cannot sustain self-renewal and pluripotency.

 What is OCT4’s involvement in pluripotency and self-renewal?

OCT4 forms a trimeric complex with SOX2 and DNA to control the expression of genes involved with embryonic development, such as FGF4 (PMID: 10801796), UTF1 (PMID: 10409735), or even NANOG (PMID: 15743839). OCT4 is critical for early embryogenesis (PMID: 9814708) and for embryonic stem cell pluripotency (PMID: 16153702). High levels of OCT4 are associated with the ability to self-renew, which is emphasized by OCT4 gene knockdowns promoting differentiation (PMID: 10742100). OCT4 is also one of the four key transcription factors used to generate induced pluripotent stem cells (iPSCs) by reprogramming fibroblasts to a pluripotent state (PMID: 16904174).




Protocols

Product Specific Protocols
WB protocol for OCT4 antibody 11263-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF,WB

Stem Cell Res

Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for the STUB1 locus.

Authors - Stefanie Schuster
humanWB

Int J Food Sci Nutr

Sulforaphane inhibits gastric cancer stem cells via suppressing sonic hedgehog pathway.

Authors - Miaomiao Ge
humanWB,IHC

Front Oncol

Scorpion Venom Analgesic Peptide, BmK AGAP Inhibits Stemness, and Epithelial-Mesenchymal Transition by Down-Regulating PTX3 in Breast Cancer.

Authors - Sylvanus Kampo
humanWB

Mol Cancer

Myeloid-derived suppressor cells endow stem-like qualities to multiple myeloma cells by inducing piRNA-823 expression and DNMT3B activation.

Authors - Lisha Ai
IF

Cell Mol Biol Lett

Limbal niche cells can reduce the angiogenic potential of cultivated oral mucosal epithelial cells.

Authors - Chao-Ye Duan
mouseWB,IHC

J Exp Clin Cancer Res

ADAR1 promotes the epithelial-to-mesenchymal transition and stem-like cell phenotype of oral cancer by facilitating oncogenic microRNA maturation.

Authors - Xue Liu