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OCT4 Antibody 50 Publications

Rabbit Polyclonal| Catalog number: 11263-1-AP

Featured Product KD/KO validated

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Con: 54 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

OCT4 Antibody IHC human colon cancer tissue 11263-1-AP

Immunohistochemical of paraffin-embedded human colon cancer using 11263-1-AP(OCT4 antibody) at dilution of 1:50 (under 10x lens)

OCT4 Antibody IF human embronic stem cells 11263-1-AP

Confocal immunofluorescent analysis of human embronic stem cells with 11263-1-AP at dilution of 1:50. The FITC shows staining with 11263-1-AP/FITC. The DAPI shows nuclear staining by DAPI. The MERGE is the merge of FITC and DAPI.

OCT4 Antibody WB H7ES cells 11263-1-AP

H7ES cells were subjected to SDS PAGE followed by western blot with 11263-1-AP( OCT4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Species specificity:
human, mouse, rat
Positive WB detected in:
H7ES cells, HEK-293 cells, hESC cells, human placenta tissue, mouse brain tissue, mouse embryo tissue, mouse kidney tissue, NCCIT cell
Positive IHC detected in:
human colon cancer tissue
Positive IF detected in:
human embronic stem cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:50-1:200
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
OCT4 fusion protein Ag1794
Full name:
POU class 5 homeobox 1
Calculated molecular weight:
39 kDa
Observed molecular weight:
50 kDa
GenBank accession number:
BC020712
Gene ID (NCBI):
5460
Gene symbol
POU5F1
Synonyms
Oct 3, Oct 4, OCT3, OCT3/4, OCT4, Octamer binding protein 3, Octamer binding protein 4, OTF 3, OTF3, OTF4, POU class 5 homeobox 1, POU5F1

Background

Expression of the Oct-4 transcription factor is associated with an undifferentiated phenotype of human and mouse embryonic stem cells and germ cells. Oct-4 (Octamer-4) is a homeodomain transcription factor of the POU family (POU5F1) involved in regulation of pluripotency during normal development and is detectable in embryonic stem (ES) and germ cells. Its expression is associated with an undifferentiated phenotype and tumors. In fact, gene knockdown of Oct4 promotes differentiation, thereby demonstrating a role for this factor in human ES cell self-renewal. As such, it is frequently used as a marker for undifferentiated ES cells or iPS (induced pluripotent stem) cells. Oct4 is one of the four key transcription factors (Oct4, Sox2, c-Myc, and KLF4) used to reprogram mouse and human fibroblasts to a pluripotent state as well as generate patient-specific iPS cells. Affinity purified rabbit anti-Oct4 can be used to demonstrate pluripotency of ES and iPS cells. The calculated molecular weight of OCT4 is 38 kDa, but the modified OCT4 protein is about 50 kDa.


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