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  • KD/KO Validated

ODF2 Polyclonal antibody

ODF2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12058-1-AP

Synonyms

Cenexin, ODF2, ODF2/1, ODF2/2, ODF84, Outer dense fiber protein 2



Tested Applications

Positive WB detected inmouse testis tissue, mouse lung tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12058-1-AP targets ODF2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ODF2 fusion protein Ag2693
Full Name outer dense fiber of sperm tails 2
Calculated molecular weight 763 aa, 89 kDa
Observed molecular weight 67-100 kDa
GenBank accession numberBC010629
Gene symbol ODF2
Gene ID (NCBI) 4957
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ODF2 (outer dense fiber 2) is the major cenexin protein of the cytoskeleton of the sperm tail. In somatic cells, it is a component of the centrosome in which it is located in the appendages of the mother centriole. The gene for the cenexin protein, which was first identified as a protein marker of the mother centriole, was subsequently found to be identical to ODF2 and encodes transcripts cenexin 1 and cenexin 2. ODF2 is ubiquitously expressed in many cell types as various splicing variants. Additionally, ODF2 localizes to centrioles, basal bodies, and primary cilia, which are all structurally and functionally interconnected. ODF2 has 10 isoforms with MW 67-100 kDa.

Protocols

Product Specific Protocols
WB protocol for ODF2 antibody 12058-1-APDownload protocol
IHC protocol for ODF2 antibody 12058-1-APDownload protocol
IF protocol for ODF2 antibody 12058-1-APDownload protocol
IP protocol for ODF2 antibody 12058-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Loss-of-function mutations in QRICH2 cause male infertility with multiple morphological abnormalities of the sperm flagella.

Authors - Ying Shen
humanIF

Nat Commun

High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy.

Authors - Mathew Bowler
humanWB,IF

Mol Biol Cell

Chromosome misalignment is associated with PLK1 activity at cenexin-positive mitotic centrosomes.

Authors - Erica G Colicino
  • KD Validated
mouseWB

Biol Reprod

Intraflagellar transport protein 74 is essential for spermatogenesis and male fertility in mice‡.

Authors - Lin Shi
ratIF

Cytoskeleton (Hoboken)

Myosin Id is required for planar cell polarity in ciliated tracheal and ependymal epithelial cells.

Authors - Peter S Hegan
MOUSEWB

Reproduction

Nicotine reduces Sord expression and tyrosine phosphorylation in capacitated sperm.

Authors - Jingbo Dai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jay (Verified Customer) (12-02-2019)

Methanol Fixation. Immunostaining result shows that Odf2 specifically marks the mother centriole in ciliated NIH3T3 cell (Odf2;red, GT335;green for primary cilia, DAPI;blue for nucleus).

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse NIH3T3
ODF2 Antibody Immunofluorescence, validation (1:200 dilution) in Mouse NIH3T3 (Cat no:12058-1-AP)