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OGT Polyclonal antibody

OGT Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11576-2-AP

Synonyms

HRNT1, OGT



Tested Applications

Positive WB detected inmouse brain tissue, mouse liver tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue, rat stomach tissue, rat testis tissue, human pancreas cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11576-2-AP targets OGT in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OGT fusion protein Ag2160
Full Name O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
Calculated molecular weight 1046 aa, 117 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC014434
Gene symbol OGT
Gene ID (NCBI) 8473
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

O-linked N-acetylglucosamine transferase (OGT) catalyzes the attachment of N-acetylglucosamine (GlcNAc) monosaccharides to the hydroxyl group of serine or threonine residues of numerous nuclear and cytoplasmic proteins and may play important roles in a large number of diverse intracellular processes ranging from translational control, transcription, transcriptional repression, INS resistance and regulation of the cell cycle. It exists as a heterotrimeric complex with two 110 kDa and one 70 kDa subunits. Recent studies have shown that O-GlcNAcylation plays essential roles in cancer formation and progression. O-GlcNAcylation as well as OGT expression was found to be significantly elevated in the cancer tissues.

Protocols

Product Specific Protocols
WB protocol for OGT antibody 11576-2-APDownload protocol
IHC protocol for OGT antibody 11576-2-APDownload protocol
IP protocol for OGT antibody 11576-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Biomed Res

O-linked N-acetylglucosamine transferase (OGT) is overexpressed and promotes O-linked protein glycosylation in esophageal squamous cell carcinoma.

Authors - Qiao Zhe Z
humanIHC

Oncotarget

High O-linked N-acetylglucosamine transferase expression predicts poor survival in patients with early stage lung adenocarcinoma.

Authors - Yi-Cheng Lin
ratIF

Korean Circ J

Glucose-insulin-potassium solution protects ventricular myocytes of neonatal rat in an in vitro coverslip ischemia/reperfusion model.

Authors - Woo-Jung Chun
mouseWB

Biol Proced Online

A Modified Coupled Enzyme Method for O-linked GlcNAc Transferase Activity Assay.

Authors - Lianwen Zhang
humanWB

Exp Ther Med

Long non-coding RNA LINC00707 acts as a competing endogenous RNA to enhance cell proliferation in colorectal cancer.

Authors - Han Wang
humanWB

Oncol Lett

Downregulation of O-linked N-acetylglucosamine transferase by RNA interference decreases MMP9 expression in human esophageal cancer cells.

Authors - Zhe Qiao
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