OGT Antibody 11576-2-AP

Tested Applications

Applications:

ELISA, IF, IHC, IP, WB
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IP Result of anti-OGT (IP:11576-2-AP, 3ug; Detection:11576-2-AP 1:1000) with mouse brain tissue lysate 8000ug.

human brain tissue were subjected to SDS PAGE followed by western blot with 11576-2-AP(OGT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Species specificity:

human, mouse, rat

Positive WB detected in:

human brain tissue, human liver tissue, mouse brain tissue, mouse liver tissue, rat brain tissue

Positive IP detected in:

mouse brain tissue

Recommended dilution:

WB : 1:500-1:3000

IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

OGT fusion protein Ag2160

Full name:

O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)

Calculated molecular weight:

1046aa,117 kDa

Observed molecular weight:

110 kDa

GenBank accession number:

BC014434

Gene ID (NCBI):

8473

Gene symbol

OGT

Synonyms

HRNT1, O GLCNAC, OGT

Background

O-linked N-acetylglucosamine transferase (OGT) catalyzes the attachment of N-acetylglucosamine (GlcNAc) monosaccharides to the hydroxyl group of serine or threonine residues of numerous nuclear and cytoplasmic proteins and may play important roles in a large number of diverse intracellular processes ranging from translational control, transcription, transcriptional repression, insulin resistance and regulation of the cell cycle. It exists as a heterotrimeric complex with two 110 kDa and one 70 kDa subunits. Recent studies have shown that O-GlcNAcylation plays essential roles in cancer formation and progression. O-GlcNAcylation as well as OGT expression was found to be significantly elevated in the cancer tissues.

Showing 1- [6] of [8] Publications

Author:		Zhu Qianqian Q
Pubmed ID:		21461968
Journal:		Med Oncol
Title:		O-GlcNAcylation plays a role in tumor recurrence of hepatocellular carcinoma following liver transplantation.
Application:		WB
KD validated
Species:		human

Author:		Zhe Qiao
Pubmed ID:		27123109
Journal:		Oncol Lett
Title:		Downregulation of O-linked N-acetylglucosamine transferase by RNA interference decreases MMP9 expression in human esophageal cancer cells.
Application:		WB
KD validated
Species:		human

Author:		Weiqi Xu
Pubmed ID:		28347804
Journal:		J Hepatol
Title:		O-GlcNAc transferase promotes fatty liver-associated liver cancer through inducing palmitic acid and activating endoplasmic reticulum stress.
Application:		WB
KD validated
Species:		human

Author:		Lianwen Zhang
Pubmed ID:		19957065
Journal:		Biol Proced Online
Title:		A Modified Coupled Enzyme Method for O-linked GlcNAc Transferase Activity Assay.
Application:		WB

Species:		mouse

Author:		Krithika Vaidyanathan
Pubmed ID:		28302723
Journal:		J Biol Chem
Title:		Identification and Characterization of a Missense Mutation in the O-GlcNAc Transferase Gene that Segregates with X-Linked Intellectual Disability.
Application:		WB

Species:		human

Author:		Qiao Zhe Z
Pubmed ID:		23554759
Journal:		J Biomed Res
Title:		O-linked N-acetylglucosamine transferase (OGT) is overexpressed and promotes O-linked protein glycosylation in esophageal squamous cell carcinoma.
Application:		IHC

Species:		human

WB Figures

human brain tissue were subjected to SDS PAGE followed by western blot with 11576-2-AP(OGT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

human liver tissue were subjected to SDS PAGE followed by western blot with 11576-2-AP(OGT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 11576-2-AP( OGT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

mouse liver tissue were subjected to SDS PAGE followed by western blot with 11576-2-AP( OGT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

rat brain tissue were subjected to SDS PAGE followed by western blot with 11576-2-AP( OGT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 11576-2-AP( OGT Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 11576-2-AP( OGT Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 11576-2-AP( OGT Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 11576-2-AP( OGT Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical of paraffin-embedded human pancreas cancer using 11576-2-AP(OGT antibody) at dilution of 1:50 (under 10x lens)

IP Figures

IP Result of anti-OGT (IP:11576-2-AP, 3ug; Detection:11576-2-AP 1:1000) with mouse brain tissue lysate 8000ug.

OGT Antibody 8 Publications

Rabbit Polyclonal| Catalog number: 11576-2-AP

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Tested Applications

Product Information

Immunogen Information

Background

Showing 1- [6] of [8] Publications

WB Figures

IHC Figures

IP Figures

OGT Antibody 8 Publications

Rabbit Polyclonal| Catalog number: 11576-2-AP

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Tested Applications

Product Information

Immunogen Information

Background