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OGT Antibody 13 Publications

Rabbit Polyclonal| Catalog number: 11576-2-AP

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Con: 55 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

OGT Antibody WB A549 cells 11576-2-AP

Various lysates were subjected to SDS PAGE followed by western blot with 11576-2-AP (OGT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

OGT Antibody IP mouse brain tissue 11576-2-AP

IP Result of anti-OGT (IP:11576-2-AP, 3ug; Detection:11576-2-AP 1:1000) with mouse brain tissue lysate 8000ug.

OGT Antibody WB human brain tissue 11576-2-AP

human brain tissue were subjected to SDS PAGE followed by western blot with 11576-2-AP(OGT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

OGT Antibody IHC human colon cancer tissue 11576-2-AP

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 11576-2-AP( OGT Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

OGT Antibody IHC human colon cancer tissue 11576-2-AP

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 11576-2-AP( OGT Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat
Positive WB detected in:
human brain tissue, human liver tissue, A549 cells, rat brain tissue, mouse brain tissue, mouse liver tissue
Positive IP detected in:
mouse brain tissue
Positive IHC detected in:
human colon cancer tissue, mouse pancreas tissue, rat stomach tissue, rat testis tissue, human pancreas cancer tissue, human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:3000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
OGT fusion protein Ag2160
Full name:
O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
Calculated molecular weight:
1046aa,117 kDa
Observed molecular weight:
110 kDa
GenBank accession number:
BC014434
Gene ID (NCBI):
8473
Gene symbol
OGT
Synonyms
HRNT1, O GLCNAC, OGT

Background

O-linked N-acetylglucosamine transferase (OGT) catalyzes the attachment of N-acetylglucosamine (GlcNAc) monosaccharides to the hydroxyl group of serine or threonine residues of numerous nuclear and cytoplasmic proteins and may play important roles in a large number of diverse intracellular processes ranging from translational control, transcription, transcriptional repression, insulin resistance and regulation of the cell cycle. It exists as a heterotrimeric complex with two 110 kDa and one 70 kDa subunits. Recent studies have shown that O-GlcNAcylation plays essential roles in cancer formation and progression. O-GlcNAcylation as well as OGT expression was found to be significantly elevated in the cancer tissues.


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