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OLR1 Polyclonal antibody

OLR1 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 11837-1-AP

Synonyms

CLEC8A, hLOX 1, Lectin like oxLDL receptor 1, LOX 1, LOX1, OLR1, Ox LDL receptor 1, SCARE1



Tested Applications

Positive WB detected inhuman liver tissue
Positive IP detected inL02 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11837-1-AP targets OLR1 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OLR1 fusion protein Ag2437
Full Name oxidized low density lipoprotein (lectin-like) receptor 1
Calculated molecular weight 273 aa, 31 kDa
Observed molecular weight 48-52 kDa
GenBank accession numberBC022295
Gene symbol OLR1
Gene ID (NCBI) 4973
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OLR1/LOX-1 acts as a receptor, in the form of homodimer, that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) (PMID: 9052782, 15695803). ORL1 is predominantly expressed in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain (PMID: 9828121). OLR1 is a protein containing 273 amino acid with the calculated molecular mass of 31 kDa but an apparent molecular mass of 50 kDa, which may result from glycosylation of four potential N-linked glycosylation sites located at C-terminal domain (PMID: 9052782).

Protocols

Product Specific Protocols
WB protocol for OLR1 antibody 11837-1-APDownload protocol
IHC protocol for OLR1 antibody 11837-1-APDownload protocol
IP protocol for OLR1 antibody 11837-1-APDownload protocol
FC protocol for OLR1 antibody 11837-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biochem Biophys Res Commun

ALDH2 deficiency inhibits Ox-LDL induced foam cell formation via suppressing CD36 expression.

Authors - Shujian Wei
humanWB

Int Immunopharmacol

Wedelolactone suppresses IL-1β maturation and neutrophil infiltration in Aspergillus fumigatus keratitis.

Authors - Min Cheng
humanWB

Sci Rep

An in vitro model of foam cell formation induced by a stretchable microfluidic device.

Authors - Xiaoyang Gu
humanIHC

Diagnostics (Basel)

Increased Tissue Expression of Lectin-Like Oxidized LDL Receptor-1 (LOX-1) Is Associated with Disease Severity in Chronic Rhinosinusitis with Nasal Polyps.

Authors - Manabu Nishida
humanWB

Molecules

Mitochondrion-Targeted Peptide SS-31 Inhibited Oxidized Low-Density Lipoproteins-Induced Foam Cell Formation through both ROS Scavenging and Inhibition of Cholesterol Influx in RAW264.7 Cells.

Authors - Shuangying Hao
mouseWB

Cornea

Fenretinide Inhibits Neutrophil Recruitment and IL-1β Production in Aspergillus fumigatus Keratitis.

Authors - Wenyi Zhao