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PCK1 Antibody

PCK1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 16754-1-AP

Synonyms

PCK1, PEPCK C, PEPCK1, PEPCKC, Phosphoenolpyruvate carboxykinase 1



Tested Applications

Positive WB detected inmouse liver tissue, HepG2 cells, rat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16754-1-AP targets PCK1 in IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species goat, human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCK1 fusion protein Ag10261
Full Name phosphoenolpyruvate carboxykinase 1 (soluble)
Calculated molecular weight 622aa,69 kDa
Observed molecular weight 66-69 kDa
GenBank accession number BC023978
Gene symbol PCK1
Gene ID (NCBI) 5105
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).

Protocols

Product Specific Protocols
WB protocol for PCK1 antibody 16754-1-APDownload protocol
IHC protocol for PCK1 antibody 16754-1-APDownload protocol
IF protocol for PCK1 antibody 16754-1-APDownload protocol
IP protocol for PCK1 antibody 16754-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EBioMedicine

Liver-derived fibroblast growth factor 21 mediates effects of glucagon-like peptide-1 in attenuating hepatic glucose output.

Authors - Junling Liu
humanWB

Diabetes Metab Syndr Obes

Resveratrol affects hepatic gluconeogenesis via histone deacetylase 4.

Authors - Hang Zhao
mouseWB

Physiol Rep

Adropin reduces blood glucose levels in mice by limiting hepatic glucose production.

Authors - Dharendra Thapa
mouseIF

J Am Soc Nephrol

Von Hippel-Lindau Acts as a Metabolic Switch Controlling Nephron Progenitor Differentiation.

Authors - Kasey Cargill
goatWB

J Anim Sci Biotechnol

Effects of chronic dexamethasone administration on hyperglycemia and insulin release in goats.

Authors - Liqiong Niu
WB

Poult Sci

Breed-dependent transcriptional regulation of phosphoenolpyruvate carboxylase, cystolic form, expression in the liver of broiler chickens.

Authors - Guo Feng F