Tested Applications
| Positive WB detected in | mouse liver tissue, rat liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16754-1-AP targets PCK1/2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, zebrafish, bovine, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10261 Product name: Recombinant human PCK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 274-622 aa of BC023978 Sequence: EGEKKYLAAAFPSACGKTNLAMMNPSLPGWKVECVGDDIAWMKFDAQGHLRAINPENGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLASGVTITSWKNKEWSSEDGEPCAHPNSRFCTPASQCPIIDAAWESPEGVPIEGIIFGGRRPAGVPLVYEALSWQHGVFVGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKYLAHWLSMAQHPAAKLPKIFHVNWFRKDKEGKFLWPGFGENSRVLEWMFNRIDGKASTKLTPIGYIPKEDALNLKGLGHINMMELFSISKEFWEKEVEDIEKYLEDQVNADLPCEIEREILALKQRISQM Predict reactive species |
| Full Name | phosphoenolpyruvate carboxykinase 1 (soluble) |
| Calculated Molecular Weight | 622 aa, 69 kDa |
| Observed Molecular Weight | 66-69 kDa |
| GenBank Accession Number | BC023978 |
| Gene Symbol | PCK1 |
| Gene ID (NCBI) | 5105 |
| RRID | AB_2160031 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35558 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD). This antibody has weak cross-reactivity with PCK2 protein due to high sequence homology between PCK1 and PCK2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PCK1/2 antibody 16754-1-AP | Download protocol |
| IP protocol for PCK1/2 antibody 16754-1-AP | Download protocol |
| WB protocol for PCK1/2 antibody 16754-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-PCK1 (PEPCK1) antibody, catalog number 16754-1-AP, has accumulated 113 citations across high-impact journals including Nature, Cell Metabolism, Nature Metabolism, Nature Communications, Molecular Cell, Journal of Experimental Medicine, and Science Advances. Research fields span hepatic gluconeogenesis, lipid metabolism, diabetes and insulin resistance, cancer biology (hepatocellular, breast, colorectal, and renal carcinomas), NAFLD/NASH, gut microbiota modulation, inflammation, and exercise physiology.
| Species | Application | Title |
|---|---|---|
Nature The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.
| ||
Cell Metab A 5:2 intermittent fasting regimen ameliorates NASH and fibrosis and blunts HCC development via hepatic PPARα and PCK1 | ||
Cancer Commun (Lond) Simvastatin overcomes the pPCK1-pLDHA-SPRINGlac axis-mediated ferroptosis and chemo-immunotherapy resistance in AKT-hyperactivated intrahepatic cholangiocarcinoma
| ||
Nat Metab Redox-dependent liver gluconeogenesis impacts different intensity exercise in mice | ||
Nat Commun Glycogen metabolism regulates macrophage-mediated acute inflammatory responses. |
Reviews
What customers say
The single review for 16754-1-AP is from James A., who rated the product 5 out of 5 stars. He used it for Western Blot at a 1/1000 dilution to detect PCK1 in liver protein extracts, and the results were successful. The review was posted in November 2022 using lot 00060917. Overall, the feedback is positive, indicating reliable performance for WB applications on liver tissue.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
James (Verified Customer) (11-18-2022) | Detection of PCK1 in liver protein extracts
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