|Positive WB detected in||mouse liver tissue, HepG2 cells, rat liver tissue|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
16754-1-AP targets PCK1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||goat, human, mouse, rat, swine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PCK1 fusion protein Ag10261|
|Full Name||phosphoenolpyruvate carboxykinase 1 (soluble)|
|Calculated molecular weight||622 aa, 69 kDa|
|Observed molecular weight||66-69 kDa|
|GenBank accession number||BC023978|
|Gene ID (NCBI)||5105|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).
|Product Specific Protocols|
|WB protocol for PCK1 antibody 16754-1-AP||Download protocol|
|IHC protocol for PCK1 antibody 16754-1-AP||Download protocol|
|IF protocol for PCK1 antibody 16754-1-AP||Download protocol|
|IP protocol for PCK1 antibody 16754-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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