PCNA Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 24036-1-AP

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Freight/Packing

Con: 37 μg/150 μl

Choose size:

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Species specificity:
human, mouse, rat

Positive WB detected in:
HEK-293 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, mouse testis tissue, NIH/3T3 cells, Raji cells, rat liver tissue

Positive IP detected in:
HEK-293 cells

Positive IHC detected in:
human breast cancer tissue, human stomach cancer tissue

Positive IF detected in:
HepG2 cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen Affinity purified

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
proliferating cell nuclear antigen

Calculated molecular weight:
29 kDa/31 kDa

Observed molecular weight:
36-38 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
PCNA

Synonyms
Cyclin, PCNA
Background

The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli; however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms: one involved in ongoing DNA replication, which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form.This antibody specifically react with the 36kd human PCNA protein.


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