PD-L2/CD273 Polyclonal antibody

PD-L2/CD273 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 18251-1-AP

Synonyms

B7 DC, Btdc, Butyrophilin B7 DC, CD273, PD 1 ligand 2, PDCD1 ligand 2, PDCD1L2, PDCD1LG2, PDL2, PD-L2, Programmed death ligand 2



Tested Applications

Positive WB detected inRAW 264.7 cells, HepG2 cells
Positive IHC detected inhuman tonsillitis tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18251-1-AP is PD-L2/CD273 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PD-L2/CD273 fusion protein Ag13039
Full Name programmed cell death 1 ligand 2
Calculated molecular weight 273 aa, 31 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC074766
Gene symbol PDCD1LG2
Gene ID (NCBI) 80380
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Programmed cell death ligand 2 (PD-L2, also known as CD273, or B7-DC), is one of the B7 family molecules which are type I transmembrane proteins belonging to the immunoglobulin superfamily. PD-L2 was identified both by its homology to PD-L1 and by analysis of genes differentially expressed in libraries generated from dendritic cells (DC) and activated macrophages (PMID: 14515254). PD-L2 is a second ligand for PD-1, a costimulatory molecule that plays an inhibitory role in regulating T cell activation in the periphery. Mouse PD-L2 has a predicted molecular weight of 28 kDa.

Protocols

Product Specific Protocols
WB protocol for PD-L2/CD273 antibody 18251-1-APDownload protocol
IHC protocol for PD-L2/CD273 antibody 18251-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Oncoimmunology

PD-L2 expression is correlated with the molecular and clinical features of glioma, and acts as an unfavorable prognostic factor.

Authors - Zhi-Liang Wang
mouseWB,IHC

Antiviral Res

Aberrant PD-1 ligand expression contributes to the myocardial inflammatory injury caused by Coxsackievirus B infection.

Authors - Tianying Wang
humanIHC

Ann Hematol

PD-L1, LAG3, and HLA-DR are increasingly expressed during smoldering myeloma progression.

Authors - Alberto Mussetti
humanIHC

Urol Oncol

Programmed death-ligand 2 (PD-L2) expression in bladder cancer.

Authors - Yubo Yang
humanIHC

J Cell Mol Med

IFI30 expression is an independent unfavourable prognostic factor in glioma.

Authors - Xiu Liu
humanIHC

Cancer Sci

ANO9 regulates PD-L2 expression and binding ability to PD-1 in gastric cancer.

Authors - Keita Katsurahara