|Positive WB detected in||RAW 264.7 cells, HepG2 cells|
|Positive IHC detected in||human tonsillitis tissue, human skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 18251-1-AP is PD-L2/CD273 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PD-L2/CD273 fusion protein Ag13039|
|Full Name||programmed cell death 1 ligand 2|
|Calculated molecular weight||273 aa, 31 kDa|
|Observed molecular weight||28 kDa|
|GenBank accession number||BC074766|
|Gene ID (NCBI)||80380|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Programmed cell death ligand 2 (PD-L2, also known as CD273, or B7-DC), is one of the B7 family molecules which are type I transmembrane proteins belonging to the immunoglobulin superfamily. PD-L2 was identified both by its homology to PD-L1 and by analysis of genes differentially expressed in libraries generated from dendritic cells (DC) and activated macrophages (PMID: 14515254). PD-L2 is a second ligand for PD-1, a costimulatory molecule that plays an inhibitory role in regulating T cell activation in the periphery. Mouse PD-L2 has a predicted molecular weight of 28 kDa.
PD-L2 expression is correlated with the molecular and clinical features of glioma, and acts as an unfavorable prognostic factor.
Aberrant PD-1 ligand expression contributes to the myocardial inflammatory injury caused by Coxsackievirus B infection.
PD-L1, LAG3, and HLA-DR are increasingly expressed during smoldering myeloma progression.
Programmed death-ligand 2 (PD-L2) expression in bladder cancer.
J Cell Mol Med
IFI30 expression is an independent unfavourable prognostic factor in glioma.
ANO9 regulates PD-L2 expression and binding ability to PD-1 in gastric cancer.