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Alix Antibody 19 Publications

Rabbit Polyclonal| Catalog number: 12422-1-AP

Featured Product KD/KO validated

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Con: 21 μg/150 μl

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Tested Applications

Applications:
WB, IP, IF, ELISA
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Images See all

Alix Antibody IP Jurkat cells 12422-1-AP

IP Result of anti-PDCD6IP (IP:12422-1-AP, 3ug; Detection:12422-1-AP 1:500) with Jurkat cells lysate 4000ug.

Alix Antibody IF Hela cells 12422-1-AP

Immunofluorescent analysis of Hela cells, using PDCD6IP antibody 12422-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Alix Antibody WB Jurkat cells 12422-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 12422-1-AP( PDCD6IP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours

Species specificity:
human, mouse, rat
Positive WB detected in:
Jurkat cells, mouse liver tissue, rat liver tissue
Positive IP detected in:
Jurkat cells
Positive IF detected in:
Hela cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Alix fusion protein Ag3074
Full name:
programmed cell death 6 interacting protein
Calculated molecular weight:
868aa,96 kDa
Observed molecular weight:
96 kDa
GenBank accession number:
BC020066
Gene ID (NCBI):
10015
Gene symbol
PDCD6IP
Synonyms
AIP1, ALG 2 interacting protein 1, Alix, DRIP4, HP95, KIAA1375, PDCD6 interacting protein, PDCD6IP

Background

ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium.


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