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  • KD/KO Validated

PDPK1 Polyclonal antibody

PDPK1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17086-1-AP

Synonyms

hPDK1, PDK1, PDPK1, PRO0461



Tested Applications

Positive WB detected in MCF-7 cells, LNCaP cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17086-1-AP targets PDPK1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDPK1 fusion protein Ag9213
Full Name 3-phosphoinositide dependent protein kinase-1
Calculated Molecular Weight 556 aa, 63 kDa
Observed Molecular Weight 60-63 kDa
GenBank Accession NumberBC012103
Gene Symbol PDPK1
Gene ID (NCBI) 5170
RRIDAB_2161289
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

3-Phosphoinositide dependent protein kinase-1 (PDK1 or PDPK1) is a serine-threonine kinase belonging to AGC kinase family. PDK1 plays a critical role in establishing ACD (Asymmetric cell division) in the epithelium (PMID: 27184845). The kinase activity of PDK1 depends on phosphatidyl inositol 3-kinase (PI3K), a key intermediate in signaling pathways including those from growth factor receptors and adhesion molecules. Substrates of PDK1, including AKT and the protein kinase C (PKC) isozymes, regulate a number of essential cell functions (PMID: 20027184). PDPK1 has 5 isoforms produced by alternative splicing of 48~63 kDa and is detected as 60-63 kDa.

Protocols

Product Specific Protocols
WB protocol for PDPK1 antibody 17086-1-APDownload protocol
IHC protocol for PDPK1 antibody 17086-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

A Non-canonical PDK1-RSK Signal Diminishes Pro-caspase-8-Mediated Necroptosis Blockade.

Authors - Zhang-Hua Yang
  • KO Validated
humanWB

PLoS Biol

Loss of hepatic aldolase B activates Akt and promotes hepatocellular carcinogenesis by destabilizing the Aldob/Akt/PP2A protein complex.

Authors - Xuxiao He
humanWB

Free Radic Biol Med

Redox sensor NPGPx restrains ZAP70 activity and modulates T cell homeostasis.

Authors - Fang-Yi Su
humanIHC

Front Immunol

Identification of senescence-related subtypes, establishment of a prognosis model, and characterization of a tumor microenvironment infiltration in breast cancer

Authors - Yanling Zhou
  • KD Validated
mouseWB

J Innate Immun

Mannan-Binding Lectin Reduces Epithelial-Mesenchymal Transition in Pulmonary Fibrosis via Inactivating the Store-Operated Calcium Entry Machinery.

Authors - Yunzhi Liu
humanWB

Biol Trace Elem Res

HIF-1α Mediates Arsenic-Induced Metabolic Reprogramming in Lung Bronchial Epithelial Cells.

Authors - Wenjuan Wang