PGAM1 Polyclonal antibody

PGAM1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16126-1-AP

Synonyms

BPG dependent PGAM 1, PGAM B, PGAM1, PGAMA, Phosphoglycerate mutase 1



Tested Applications

Positive WB detected inA549 cells, human brain tissue, HepG2 cells, rat brain tissue, rat liver tissue, MCF-7 cells, mouse liver tissue, HEK-293T cells, HeLa cells, Jurkat cells, NIH/3T3 cells, CHO cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 5 publications below

Product Information

16126-1-AP targets PGAM1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PGAM1 fusion protein Ag9110
Full Name phosphoglycerate mutase 1 (brain)
Calculated molecular weight 254 aa, 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC011678
Gene symbol PGAM1
Gene ID (NCBI) 5223
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PGAM1(phosphoglycerate mutase 1) is also named as PGAMA,PGAM-B and belongs to the phosphoglycerate mutase family. Phosphoglycerate mutase is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The homodimer PGAM1 is expressed mainly in liver, kidney, brain and overexpressed in a variety of human cancers, including breast carcinoma, colorectal cancer, lung cancer, prostate cancer, oral squamous cell carcinoma, esophageal squamous cell carcinomas and also associated with certain virus infection. PGAM1 could be developed as a useful diagnostic biomarker, as well as a potential therapeutic target for hepatocellular carcinoma (PMID:20403181). This antibody may also recognize PGAM2 and PGAM4 due to the high homology.

Protocols

Product Specific Protocols
WB protocol for PGAM1 antibody 16126-1-APDownload protocol
IHC protocol for PGAM1 antibody 16126-1-APDownload protocol
IF protocol for PGAM1 antibody 16126-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Sci

PMID: 32279388

Authors - Luyuan Ma
humanWB

Biochem Pharmacol

PMID: 32535103

Authors - Qi Feng
mouseWB

EMBO J

Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.

Authors - Lihua Wang
humanWB

Cell Res

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Authors - Linchong Sun
humanWB

Cancer Cell

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Authors - Ling Li