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Placental Growth Factor Antibody 5 Publications

Rabbit Polyclonal| Catalog number: 10642-1-AP

Featured Product KD/KO validated

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Con: 37 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

Placental Growth Factor Antibody IP human placenta tissue 10642-1-AP

IP result of anti-Placental Growth Factor(IP:10642-1-AP, 4ug; Detection:10642-1-AP 1:1000) with human placenta tissue lysate 2600 ug

Placental Growth Factor Antibody IHC human placenta tissue 10642-1-AP

Immunohistochemistry of paraffin-embedded human placenta tissue slide using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Placental Growth Factor Antibody IHC human placenta tissue 10642-1-AP

Immunohistochemistry of paraffin-embedded human placenta tissue slide using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat
Positive IP detected in:
human placenta tissue
Positive IHC detected in:
human placenta tissue, human meningioma tissue, human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Placental Growth Factor fusion protein Ag0969
Full name:
placental growth factor
Calculated molecular weight:
25 kDa
Observed molecular weight:
50 kDa
GenBank accession number:
BC007789
Gene ID (NCBI):
5228
Gene symbol
PGF
Synonyms
D12S1900, PGF, PGFL, PIGF, Placenta growth factor, placental growth factor, PLGF, PlGF 2, SHGC 10760

Background

Placenta growth factor (PLGF) is a homodimeric glycoprotein, 46-50 kDa in size, belonging to the vascular endothelial growth factor (VEGF) sub-family. It plays an important role in pathological VEGF-driven angiogenesis. PLGF is expressed not only in placental cells but also in colon and mammary carcinomas. It acts as a primary inflammatory instigator of atherosclerotic plaque instability and thus may be useful as a risk-predicting biomarker in patients with acute coronary syndromes (ACS).


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