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  • Featured Product
  • KD/KO Validated

PI4KA Polyclonal antibody

PI4KA Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12411-1-AP

Synonyms

PI4 kinase alpha, PI4K ALPHA, pi4K230, PI4KA, PIK4, PIK4CA, PtdIns 4 kinase alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PI4KA Polyclonal antibody (12411-1-AP)

    WB analysis of MCF-7 using 12411-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH-3T3 cells (RNAi) using PI4KA Polyclonal antibody (12411-1-AP)

    WB analysis of NIH-3T3 cells (RNAi) using 12411-1-AP

    WB result of anti- PI4KA (12411-1-AP) with NIH-3T3 cells (RNAi).

  • Western Blot (WB) analysis of various lysates using PI4KA Polyclonal antibody (12411-1-AP)

    WB analysis using 12411-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PI4KA Polyclonal antibody (12411-1-AP)

    WB analysis of human brain using 12411-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PI4KA Polyclonal antibody (12411-1-AP)

    WB analysis of mouse brain using 12411-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PI4KA Polyclonal antibody (12411-1-AP)

    WB analysis of HeLa using 12411-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PI4KA Polyclonal antibody (12411-1-AP)

    WB analysis of mouse brain using 12411-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PI4KA Polyclonal antibody (12411-1-AP)

    IP experiment of mouse brain using 12411-1-AP

    IP result of anti-PI4KA (IP:12411-1-AP, 4ug; Detection:12411-1-AP 1:3000) with mouse brain tissue lysate 1080 ug.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PI4KA Polyclonal antibody (12411-1-AP)

    IHC staining of human placenta using 12411-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12411-1-AP (PI4KA Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PI4KA Polyclonal antibody (12411-1-AP)

    IHC staining of human placenta using 12411-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12411-1-AP (PI4KA Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PI4KA Polyclonal antibody (12411-1-AP)

    IF Staining of MCF-7 using 12411-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PI4KA antibody (12411-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, human brain tissue, MCF-7 cells, NIH-3T3 cells (RNAi), rat brain tissue
Positive IP detected inmouse brain tissue
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12411-1-AP targets PI4KA in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PI4KA fusion protein Ag3062
Full Name phosphatidylinositol 4-kinase, catalytic, alpha
Calculated Molecular Weight 2044 aa, 231 kDa
Observed Molecular Weight 231 kDa
GenBank Accession NumberBC018120
Gene Symbol PI4KA
Gene ID (NCBI) 5297
RRIDAB_2268237
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PI4KA, also named as PIK4, PIK4CA, belongs to the PI3/PI4-kinase family and Type III PI4K subfamily. It acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate. PI4KA plays a role in the formation of membrane complexes where HCV replication takes place. (PMID:19605471).

Protocols

Product Specific Protocols
WB protocol for PI4KA antibody 12411-1-APDownload protocol
IHC protocol for PI4KA antibody 12411-1-APDownload protocol
IF protocol for PI4KA antibody 12411-1-APDownload protocol
IP protocol for PI4KA antibody 12411-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

A phosphoinositide signalling pathway mediates rapid lysosomal repair

Authors - Jay Xiaojun Tan
  • KD Validated
View Article
humanWB

Science

Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.

Authors - Shun Nagashima
  • KD Validated
View Article
humanWB,IF

Brain

Biallelic PI4KA variants cause a novel neurodevelopmental syndrome with hypomyelinating leukodystrophy.

Authors - Edgard Verdura
View Article
humanIF

EMBO J

Defining the proximal interaction networks of Arf GTPases reveals a mechanism for the regulation of PLD1 and PI4KB.

Authors - Zhengming Wu
View Article
human,mouseWB,IHC,IF

Cell Rep

A Forward Genetic Screen Targeting the Endothelium Reveals a Regulatory Role for the Lipid Kinase Pi4ka in Myelo- and Erythropoiesis.

Authors - Safiyyah Ziyad
  • KD Validated
View Article
humanWB

Biomaterials

Nrf-2-driven long noncoding RNA ODRUL contributes to modulating silver nanoparticle-induced effects on erythroid cells.

Authors - Ming Gao
  • KD Validated
View Article