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WB analysis of MCF-7 using 12411-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 12411-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of NIH-3T3 cells (RNAi) using 12411-1-AP
WB result of anti- PI4KA (12411-1-AP) with NIH-3T3 cells (RNAi).
WB analysis of human brain using 12411-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 12411-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 12411-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12411-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 12411-1-AP
IP result of anti-PI4KA (IP:12411-1-AP, 4ug; Detection:12411-1-AP 1:3000) with mouse brain tissue lysate 1080 ug.
IHC staining of human placenta using 12411-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12411-1-AP (PI4KA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 12411-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12411-1-AP (PI4KA Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 12411-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12411-1-AP (PI4KA Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of MCF-7 using 12411-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PI4KA antibody (12411-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

PI4KA Polyclonal antibody

Name: PI4KA Polyclonal antibody
Brand: Proteintech
SKU: 12411-1-AP
Rating: 5.00 (1 reviews)

PI4KA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12411-1-AP

Publications(12)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EC:2.7.1.67, Phosphatidylinositol 4-kinase alpha, Phosphatidylinositol 4-Kinase III alpha, PI4K-alpha, PI4-kinase alpha

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (12)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, mouse brain tissue, human brain tissue, MCF-7 cells, NIH-3T3 cells (RNAi), rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 8 publications below
WB	See 10 publications below
IHC	See 1 publications below
IF	See 3 publications below

Product Information

12411-1-AP targets PI4KA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3062 Product name: Recombinant human PI4KA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1745-2044 aa of BC018120 Sequence: YLAKFKVKRCGVSELEKEGLRCRSDSEDECSTQEADGQKISWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFIMKVIQSCFLSNRSRTYDMIQYYQNDIPY Predict reactive species
Full Name	phosphatidylinositol 4-kinase, catalytic, alpha
Calculated Molecular Weight	2044 aa, 231 kDa
Observed Molecular Weight	231 kDa
GenBank Accession Number	BC018120
Gene Symbol	PI4KA
Gene ID (NCBI)	5297
RRID	AB_2268237
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P42356
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PI4KA, also named as PIK4, PIK4CA, belongs to the PI3/PI4-kinase family and Type III PI4K subfamily. It acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate. PI4KA plays a role in the formation of membrane complexes where HCV replication takes place. (PMID:19605471).

Protocols

Product Specific Protocols
IF protocol for PI4KA antibody 12411-1-AP	Download protocol
IHC protocol for PI4KA antibody 12411-1-AP	Download protocol
IP protocol for PI4KA antibody 12411-1-AP	Download protocol
WB protocol for PI4KA antibody 12411-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature A phosphoinositide signalling pathway mediates rapid lysosomal repair Authors - Jay Xiaojun Tan KD Validated View Article
human	WB	Science Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division. Authors - Shun Nagashima KD Validated View Article
human	WB,IF	Brain Biallelic PI4KA variants cause a novel neurodevelopmental syndrome with hypomyelinating leukodystrophy. Authors - Edgard Verdura View Article
human	IF	EMBO J Defining the proximal interaction networks of Arf GTPases reveals a mechanism for the regulation of PLD1 and PI4KB. Authors - Zhengming Wu View Article
mouse,human	WB,IHC,IF	Cell Rep A Forward Genetic Screen Targeting the Endothelium Reveals a Regulatory Role for the Lipid Kinase Pi4ka in Myelo- and Erythropoiesis. Authors - Safiyyah Ziyad KD Validated View Article
human	WB	Biomaterials Nrf-2-driven long noncoding RNA ODRUL contributes to modulating silver nanoparticle-induced effects on erythroid cells. Authors - Ming Gao KD Validated View Article