|Positive WB detected in||mouse brain tissue, human brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human prostate cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10983-2-AP targets PICK1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PICK1 fusion protein Ag1443|
|Full Name||protein interacting with PRKCA 1|
|Calculated molecular weight||47 kDa|
|Observed molecular weight||50-55 kDa|
|GenBank accession number||BC017561|
|Gene ID (NCBI)||9463|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Protein interacting with C kinase 1 (PICK1) was first cloned as a PKC-binding partner through yeast two hybrid system. PICK1 acts as a critical regulator of membrane receptors’ subcellular trafficking to modulate neural processes such as learning and memory, and is widely expressed in brain, testis, heart, lung, liver, kidney and muscle. It probably binds to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence, for instance, PICK1 is a critical mediator of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) trafficking in neural synapses. PICK1 expression on D-serine release and glutamate transport in astrocytes suggests a potential implication of PICK1 in the progression of amyotrophic lateral sclerosis (ALS). PICK1 may also participate in breast cancer development through inhibition of TGF-β signaling.
|Product Specific Protocols|
|WB protocol for PICK1 antibody 10983-2-AP||Download protocol|
|IHC protocol for PICK1 antibody 10983-2-AP||Download protocol|
|IF protocol for PICK1 antibody 10983-2-AP||Download protocol|
|IP protocol for PICK1 antibody 10983-2-AP||Download protocol|
|Click here to view our Standard Protocols|
A taspine derivative supresses Caco-2 cell growth by competitively targeting EphrinB2 and regulating its pathway.
Berberine inhibits the proliferation and migration of breast cancer ZR-75-30 cells by targeting Ephrin-B2.
Protein interacting with C alpha kinase 1 (PICK1) is involved in promoting tumor growth and correlates with poor prognosis of human breast cancer.
Deletion of KIBRA, protein expressed in kidney and brain, increases filopodial-like long dendritic spines in neocortical and hippocampal neurons in vivo and in vitro.
PICK1 promotes caveolin-dependent degradation of TGF-β type I receptor.