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  • KD/KO Validated

PICK1 Polyclonal antibody

PICK1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10983-2-AP

Synonyms

PICK, PICK1, PRKCA binding protein, PRKCABP



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman prostate cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10983-2-AP targets PICK1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PICK1 fusion protein Ag1443
Full Name protein interacting with PRKCA 1
Calculated molecular weight 47 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC017561
Gene symbol PICK1
Gene ID (NCBI) 9463
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein interacting with C kinase 1 (PICK1) was first cloned as a PKC-binding partner through yeast two hybrid system. PICK1 acts as a critical regulator of membrane receptors’ subcellular trafficking to modulate neural processes such as learning and memory, and is widely expressed in brain, testis, heart, lung, liver, kidney and muscle. It probably binds to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence, for instance, PICK1 is a critical mediator of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) trafficking in neural synapses. PICK1 expression on D-serine release and glutamate transport in astrocytes suggests a potential implication of PICK1 in the progression of amyotrophic lateral sclerosis (ALS). PICK1 may also participate in breast cancer development through inhibition of TGF-β signaling.

Protocols

Product Specific Protocols
WB protocol for PICK1 antibody 10983-2-APDownload protocol
IHC protocol for PICK1 antibody 10983-2-APDownload protocol
IF protocol for PICK1 antibody 10983-2-APDownload protocol
IP protocol for PICK1 antibody 10983-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Oncol Rep

A taspine derivative supresses Caco-2 cell growth by competitively targeting EphrinB2 and regulating its pathway.

Authors - Bingling Dai
humanWB

Phytomedicine

Berberine inhibits the proliferation and migration of breast cancer ZR-75-30 cells by targeting Ephrin-B2.

Authors - Weina Ma
humanWB,IHC

Cancer Sci

Protein interacting with C alpha kinase 1 (PICK1) is involved in promoting tumor growth and correlates with poor prognosis of human breast cancer.

Authors - Zhang Bin B
mouseIF

Front Neuroanat

Deletion of KIBRA, protein expressed in kidney and brain, increases filopodial-like long dendritic spines in neocortical and hippocampal neurons in vivo and in vitro.

Authors - Anja Blanque
humanWB,IHC

Cell Res

PICK1 promotes caveolin-dependent degradation of TGF-β type I receptor.

Authors - Zhao Bing B
  • KO Validated