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  • KD/KO Validated

PIK3C2A Polyclonal antibody

PIK3C2A Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22028-1-AP

Synonyms

CPK, PI 3 Kinase Class 2A, PI 3-kinase C2alpha, PI3 K C2(ALPHA), PI3 K C2A, PI3K C2 alpha, PIK3C2A



Tested Applications

Positive WB detected inHeLa cells, PC-3 cells, human placenta tissue
Positive IP detected inPC-3 cells
Positive IHC detected inmouse ovary tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22028-1-AP is PIK3C2A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIK3C2A fusion protein Ag16894
Full Name phosphoinositide-3-kinase, class 2, alpha polypeptide
Calculated Molecular Weight 1686 aa, 191 kDa
Observed Molecular Weight 191 kDa
GenBank Accession NumberBC113658
Gene Symbol PIK3C2A
Gene ID (NCBI) 5286
RRIDAB_11183760
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PIK3C2A antibody 22028-1-APDownload protocol
IHC protocol for PIK3C2A antibody 22028-1-APDownload protocol
IF protocol for PIK3C2A antibody 22028-1-APDownload protocol
IP protocol for PIK3C2A antibody 22028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Science

PI(3,4)P2-mediated cytokinetic abscission prevents early senescence and cataract formation.

Authors - Federico Gulluni
  • KD Validated
humanWB, IF

Cancer Cell

Mitotic Spindle Assembly and Genomic Stability in Breast Cancer Require PI3K-C2α Scaffolding Function.

Authors - Federico Gulluni
ratWB,IF

Cell Res

Phosphatidylinositol 3,4-bisphosphate regulates neurite initiation and dendrite morphogenesis via actin aggregation.

Authors - Shu-Xin Zhang
humanWB,IF

J Cell Sci

Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization

Authors - Ellis L Ryan
  • KD Validated
humanIF, WB

J Cell Sci

Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization.

Authors - Ellis L Ryan
humanWB

Front Cell Infect Microbiol

The Role of Tim-3 on dNK Cells Dysfunction During Abnormal Pregnancy With Toxoplasma gondii Infection.

Authors - Teng Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

HUAYI (Verified Customer) (01-17-2020)

This antibody provide brilliant Western Blot results, and as well, the specificity for Immunofloruorence is high. But we are glad if more discounts would be received.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: cancer cell lines
PIK3C2A Antibody Western Blot,Immunofluorescence, validation (1:1000 dilution) in cancer cell lines (Cat no:22028-1-AP)