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VPS34 (C terminal) Polyclonal antibody

VPS34 (C terminal) Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12452-1-AP

Synonyms

hVps34, PI 3 Kinase Class 3, PI 3-kinase p100, PI3K p100, PI3K type 3, PIK3C3, PtdIns 3 kinase type 3, VPS34



Tested Applications

Positive WB detected inPC-3 cells, mouse brain tissue, HeLa cells, human testis tissue, human brain tissue, mouse lung tissue, mouse testis tissue, Jurkat cells, rat brain tissue
Positive IHC detected inhuman prostate cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12452-1-AP targets VPS34 (C terminal) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS34 (C terminal) fusion protein Ag3110
Full Name phosphoinositide-3-kinase, class 3
Calculated molecular weight 887 aa, 100 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC033004
Gene symbol PIK3C3
Gene ID (NCBI) 5289
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PIK3C3(Phosphatidylinositol 3-kinase catalytic subunit type 3) is also named as VPS34 and belongs to the PI3/PI4-kinase family.It is a catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes.It can form a dimer(PMID:20339072).

Protocols

Product Specific Protocols
WB protocol for VPS34 (C terminal) antibody 12452-1-APDownload protocol
IHC protocol for VPS34 (C terminal) antibody 12452-1-APDownload protocol
IF protocol for VPS34 (C terminal) antibody 12452-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Am J Transl Res

Protective effects of resveratrol on random-pattern skin flap survival: an experimental study.

Authors - Jinti Lin
ratWB

Cell Death Dis

FGF21 augments autophagy in random-pattern skin flaps via AMPK signaling pathways and improves tissue survival.

Authors - Kailiang Zhou
mouseWB,IF

J Thromb Haemost

Vps34 derived phosphatidylinositol 3-monophosphate modulates megakaryocyte maturation and proplatelet production through late endosomes/lysosomes.

Authors - Ivana Bertović
mouseWB

Exp Cell Res

TGF-β1 inhibits the autophagy of podocytes by activating mTORC1 in IgA nephropathy.

Authors - Xing Mao
ratWB

Am J Transl Res

Hydroxysafflor yellow A promotes multiterritory perforating flap survival: an experimental study.

Authors - Chenxi Zhang
mouseWB

Front Pharmacol

Betulinic Acid Enhances the Viability of Random-Pattern Skin Flaps by Activating Autophagy.

Authors - Jiafeng Li