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PI3K p85 alpha Antibody 25 Publications

Mouse Monoclonal| Catalog number: 60225-1-Ig |CloneNo.: 4G3C11

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Con: 225 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, ELISA
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Images See all

PI3K p85 alpha Antibody WB A549 cells 60225-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours

PI3K p85 alpha Antibody IP RAW 264.7 cells 60225-1-Ig

IP Result of anti-PI3K p85(alpha) (IP:60225-1-Ig, 5ug; Detection:60225-1-Ig 1:500) with RAW 264.7 cells lysate 1480ug.

Species specificity:
human, mouse, pig, rat, dog
Positive WB detected in:
A549 cells, PC-12 cells, mouse brain tissue, rat brain tissue, HEK-293 cells, pig brain tissue, fetal human brain tissue, Jurkat cells, Neuro-2a cells
Positive IP detected in:
RAW 264.7 cells
Recommended dilution:
WB : 1:5000-1:20000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2a
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
PI3K p85 alpha fusion protein Ag2344
Full name:
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
Calculated molecular weight:
85 kDa
Observed molecular weight:
85 kDa
GenBank accession number:
BC030815
Gene ID (NCBI):
5295
Gene symbol
PIK3R1
Synonyms
GRB1, p85, p85 ALPHA, PI 3 Kinase p85 alpha, PI 3-kinase p85α, PI3 kinase subunit p85 alpha, PI3K p85(alpha), PI3K regulatory subunit alpha, PIK3R1

Background

Phosphatidylinositol 3-kinase (PI3K) plays an important role in the metabolic actions of insulin and is required for adipogenesis. The PI3K pathway has also been identified as an important player in cancer development and progression. The Class IA PI3K heterodimer, which is composed of a P110 catalytic subunit and a P85 regulatory subunit, is activated upon association of the P85 subunit with upstream adaptor proteins or receptor tyrosine kinases. Mutations in PIK3R1 are implicated in cases of breast cancer and associated to SHORT syndrome.


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