PI3 Kinase p85 Alpha Monoclonal antibody
PI3 Kinase p85 Alpha Monoclonal Antibody for IP, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, pig, rat and More (1)
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
4G3C11
Cat no : 60225-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, PC-12 cells, mouse brain tissue, rat brain tissue, HEK-293 cells, pig brain tissue, fetal human brain tissue, Jurkat cells, Neuro-2a cells |
| Positive IP detected in | RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 36 publications below |
| IHC | See 2 publications below |
Product Information
The immunogen of 60225-1-Ig is PI3 Kinase p85 Alpha Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, pig, rat |
| Cited Reactivity | canine, human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PI3 Kinase p85 Alpha fusion protein Ag2344 |
| Full Name | phosphoinositide-3-kinase, regulatory subunit 1 (alpha) |
| Calculated molecular weight | 85 kDa |
| Observed molecular weight | 85 kDa |
| GenBank accession number | BC030815 |
| Gene symbol | PIK3R1 |
| Gene ID (NCBI) | 5295 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Phosphatidylinositol 3-kinase (PI3K) plays an important role in the metabolic actions of INS and is required for adipogenesis. The PI3K pathway has also been identified as an important player in cancer development and progression. The Class IA PI3K heterodimer, which is composed of a P110 catalytic subunit and a P85 regulatory subunit, is activated upon association of the P85 subunit with upstream adaptor proteins or receptor tyrosine kinases. Mutations in PIK3R1 are implicated in cases of breast cancer and associated to SHORT syndrome.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PI3 Kinase p85 Alpha antibody 60225-1-Ig | Download protocol |
| IHC protocol for PI3 Kinase p85 Alpha antibody 60225-1-Ig | Download protocol |
| IP protocol for PI3 Kinase p85 Alpha antibody 60225-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Pharm Pharmacol Minimally modified low-density lipoprotein upregulates the ETB and α1 receptors in mouse mesenteric arteries in vivo by activating the PI3K/Akt pathway. | ||
Cell Death Differ Glutathione S-transferases P1 protects breast cancer cell from adriamycin-induced cell death through promoting autophagy. | ||
Evid Based Complement Alternat Med Tetrandrine-Induced Autophagy in MDA-MB-231 Triple-Negative Breast Cancer Cell through the Inhibition of PI3K/AKT/mTOR Signaling. | ||
Inflammation TNF-α Regulates ITGβ1 and SYND4 Expression in Nucleus Pulposus Cells: Activation of FAK/PI3K Signaling. | ||
J Diabetes Res Hyperinsulinemia Can Cause Kidney Disease in the IGT Stage of OLETF Rats via the INS/IRS-1/PI3-K/Akt Signaling Pathway. | ||
Ann Transl Med Catalpol induces cell activity to promote axonal regeneration via the PI3K/AKT/mTOR pathway in vivo and in vitro stroke model. |
