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PIPPIN Polyclonal antibody

PIPPIN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25013-1-AP

Synonyms

CSDC2, PIPPIN, RNA binding protein PIPPin

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using PIPPIN Polyclonal antibody (25013-1-AP)

    WB analysis of mouse brain using 25013-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25013-1-AP (PIPPIN antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PIPPIN Polyclonal antibody (25013-1-AP)

    IHC staining of human kidney using 25013-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25013-1-AP (PIPPIN antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PIPPIN Polyclonal antibody (25013-1-AP)

    IHC staining of human kidney using 25013-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25013-1-AP (PIPPIN antibody at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25013-1-AP is PIPPIN Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIPPIN fusion protein Ag16216
Full Name cold shock domain containing C2, RNA binding
Calculated Molecular Weight 153 aa, 17 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC067113
Gene Symbol CSDC2
Gene ID (NCBI) 27254
RRIDAB_2879845
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSDC2 also termed as PIPPIN or RNA binding protein PIPPin is a 153 amino acid protein, which contains 1 CSD domain. CSDC2 may play a central role in the negative regulation of variant synthesis in the developing brain. CSDC2 modification in response to extracellular stimuli may modulate the regulation of protein synthesis.

Protocols

Product Specific Protocols
WB protocol for PIPPIN antibody 25013-1-APDownload protocol
IHC protocol for PIPPIN antibody 25013-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols