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PLD1 Polyclonal antibody

PLD1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 18355-1-AP

Synonyms

Choline phosphatase 1, hPLD1, Phospholipase D1, PLD 1, PLD1

Validation Data Gallery

  • WB analysis of HeLa using 18355-1-AP

    WB analysis of HeLa using 18355-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18355-1-AP (PLD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 18355-1-AP

    IP experiment of HeLa using 18355-1-AP

    IP Result of anti-PLD1 (IP:18355-1-AP, 4ug; Detection:18355-1-AP 1:500) with HeLa cells lysate 1200ug.

  • IF Staining of HeLa using 18355-1-AP

    IF Staining of HeLa using 18355-1-AP

    Immunofluorescent analysis of HeLa cells using 18355-1-AP (PLD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

18355-1-AP targets PLD1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLD1 fusion protein Ag13227
Full Name phospholipase D1, phosphatidylcholine-specific
Calculated molecular weight 1074 aa, 124 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC068976
Gene symbol PLD1
Gene ID (NCBI) 5337
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLD1(Phospholipase D1) belongs to the phospholipase D family. It is implicated in the release process of a variety of hormones, most likely at a late stage of exocytosis through the production of lipids that are essential for the fusion process and seems to be also essential for fast neurotransmitter release. PLD1 is primarily localized in vesicular structures such as endosomes, lysosomes and autophagosomes.

Publications

SpeciesApplicationTitle
humanWB,IF

J Cell Biol

Class III PI-3-kinase activates phospholipase D in an amino acid-sensing mTORC1 pathway.

Authors - Yoon Mee-Sup MS
View Article