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PLD1 Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 18355-1-AP

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Con: 46 μg/150 μl

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Tested Applications

Applications:
WB, IP, IF, ELISA
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Images See all

PLD1 Antibody WB HeLa cells 18355-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 18355-1-AP(PLD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

PLD1 Antibody IP HeLa cells 18355-1-AP

IP Result of anti-PLD1 (IP:18355-1-AP, 4ug; Detection:18355-1-AP 1:500) with HeLa cells lysate 1200ug.

PLD1 Antibody IF HeLa cells 18355-1-AP

Immunofluorescent analysis of HeLa cells using 18355-1-AP(PLD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
HeLa cells
Positive IP detected in:
HeLa cells
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
PLD1 fusion protein Ag13227
Full name:
phospholipase D1, phosphatidylcholine-specific
Calculated molecular weight:
1074aa,124 kDa
Observed molecular weight:
120 kDa
GenBank accession number:
BC068976
Gene ID (NCBI):
5337
Gene symbol
PLD1
Synonyms
Choline phosphatase 1, hPLD1, Phospholipase D1, PLD 1, PLD1

Background

PLD1(Phospholipase D1) belongs to the phospholipase D family. It is implicated in the release process of a variety of hormones, most likely at a late stage of exocytosis through the production of lipids that are essential for the fusion process and seems to be also essential for fast neurotransmitter release. PLD1 is primarily localized in vesicular structures such as endosomes, lysosomes and autophagosomes.


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