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PLK4 Antibody 10 Publications

Rabbit Polyclonal| Catalog number: 12952-1-AP

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Con: 32 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

PLK4 Antibody WB mouse lung tissue 12952-1-AP

mouse lung tissue were subjected to SDS PAGE followed by western blot with 12952-1-AP(PLK4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

PLK4 Antibody IHC human testis tissue 12952-1-AP

Immunohistochemical analysis of paraffin-embedded human testis using 12952-1-AP(PLK4 antibody) at dilution of 1:200 (under 10x lens)

PLK4 Antibody IHC human testis tissue 12952-1-AP

Immunohistochemical analysis of paraffin-embedded human testis using 12952-1-AP(PLK4 antibody) at dilution of 1:200 (under 40x lens)

PLK4 Antibody IF HeLa cells 12952-1-AP

Immunofluorescent analysis of HeLa cells using 12952-1-AP(PLK4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse lung tissue, A549 cells, MCF-7 cells
Positive IHC detected in:
human testis tissue, human cervical cancer tissue, human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:100-1:400
IF : 1:25-1:100
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
PLK4 fusion protein Ag3605
Full name:
polo-like kinase 4 (Drosophila)
Calculated molecular weight:
970aa,109 kDa
Observed molecular weight:
105-112 kDa
GenBank accession number:
BC036023
Gene ID (NCBI):
10733
Gene symbol
PLK4
Synonyms
PLK 4, PLK4, Polo like kinase 4, SAK, STK18

Background

PLK4(Polo-like kinase 4) is also named as SAK, STK18 and belongs to the Ser/Thr protein kinase family.It plays a central role in centriole duplication and a kinase implicated in tumor development, which is an upstream regulator of canonical biogenesis necessary for centriole formation.It has 3 isoforms produced by alternative splicing.


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