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PLK4 Polyclonal antibody

PLK4 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12952-1-AP

Synonyms

PLK 4, PLK4, Polo like kinase 4, SAK, STK18



Tested Applications

Positive WB detected inmouse lung tissue, A549 cells, MCF-7 cells
Positive IHC detected inhuman testis tissue, human cervical cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:25-1:100
Sample-dependent, check data in validation data gallery

Product Information

12952-1-AP targets PLK4 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLK4 fusion protein Ag3605
Full Name polo-like kinase 4 (Drosophila)
Calculated molecular weight 970 aa, 109 kDa
Observed molecular weight 105-112 kDa
GenBank accession numberBC036023
Gene symbol PLK4
Gene ID (NCBI) 10733
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLK4(Polo-like kinase 4) is also named as SAK, STK18 and belongs to the Ser/Thr protein kinase family.It plays a central role in centriole duplication and a kinase implicated in tumor development, which is an upstream regulator of canonical biogenesis necessary for centriole formation.It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PLK4 antibody 12952-1-APDownload protocol
IHC protocol for PLK4 antibody 12952-1-APDownload protocol
IF protocol for PLK4 antibody 12952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancers (Basel)

POLQ Overexpression Is Associated with an Increased Somatic Mutation Load and PLK4 Overexpression in Lung Adenocarcinoma.

Authors - Kazuya Shinmura
humanWB

Eur Rev Med Pharmacol Sci

LncRNA SNHG1 contributes to tumorigenesis and mechanism by targeting miR-338-3p to regulate PLK4 in human neuroblastoma.

Authors - N Zhang
ratWB,IF

Int J Mol Med

MicroRNA‑126 suppresses the proliferation and migration of endothelial cells in experimental diabetic retinopathy by targeting polo‑like kinase 4.

Authors - Yarong Zheng
humanWB,IHC

Int J Oncol

High PLK4 expression promotes tumor progression and induces epithelial‑mesenchymal transition by regulating the Wnt/β‑catenin signaling pathway in colorectal cancer.

Authors - Zhibin Liao
humanIHC

Front Mol Biosci

Characteristic Analysis of Featured Genes Associated With Stemness Indices in Colorectal Cancer.

Authors - Yongqu Lu
mouseWB

Mol Cell Biol

S6 Kinase- and β-TrCP2-Dependent Degradation of p19Arf Is Required for Cell Proliferation.

Authors - Tadashi Nakagawa