|Positive WB detected in||mouse lung tissue, A549 cells, MCF-7 cells|
|Positive IHC detected in||human testis tissue, human cervical cancer tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:25-1:100|
|Sample-dependent, check data in validation data gallery|
12952-1-AP targets PLK4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PLK4 fusion protein Ag3605|
|Full Name||polo-like kinase 4 (Drosophila)|
|Calculated molecular weight||970 aa, 109 kDa|
|Observed molecular weight||105-112 kDa|
|GenBank accession number||BC036023|
|Gene ID (NCBI)||10733|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PLK4(Polo-like kinase 4) is also named as SAK, STK18 and belongs to the Ser/Thr protein kinase family.It plays a central role in centriole duplication and a kinase implicated in tumor development, which is an upstream regulator of canonical biogenesis necessary for centriole formation.It has 3 isoforms produced by alternative splicing.
POLQ Overexpression Is Associated with an Increased Somatic Mutation Load and PLK4 Overexpression in Lung Adenocarcinoma.
Eur Rev Med Pharmacol Sci
LncRNA SNHG1 contributes to tumorigenesis and mechanism by targeting miR-338-3p to regulate PLK4 in human neuroblastoma.
Int J Oncol
High PLK4 expression promotes tumor progression and induces epithelial‑mesenchymal transition by regulating the Wnt/β‑catenin signaling pathway in colorectal cancer.
Mol Cell Biol
S6 Kinase- and β-TrCP2-Dependent Degradation of p19Arf Is Required for Cell Proliferation.
Eur J Hum Genet
Novel compound heterozygous variants in PLK4 identified in a patient with autosomal recessive microcephaly and chorioretinopathy.
Male hypogonadism and germ cell loss caused by a mutation in Polo-like kinase 4.