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- KD/KO Validated
PLK4 Polyclonal antibody
PLK4 Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 12952-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse lung tissue, A549 cells, MCF-7 cells |
Positive IHC detected in | human testis tissue, human cervical cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF) | IF : 1:25-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 10 publications below |
IHC | See 6 publications below |
IF | See 1 publications below |
Product Information
12952-1-AP targets PLK4 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PLK4 fusion protein Ag3605 |
Full Name | polo-like kinase 4 (Drosophila) |
Calculated molecular weight | 970 aa, 109 kDa |
Observed molecular weight | 105-112 kDa |
GenBank accession number | BC036023 |
Gene symbol | PLK4 |
Gene ID (NCBI) | 10733 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PLK4(Polo-like kinase 4) is also named as SAK, STK18 and belongs to the Ser/Thr protein kinase family.It plays a central role in centriole duplication and a kinase implicated in tumor development, which is an upstream regulator of canonical biogenesis necessary for centriole formation.It has 3 isoforms produced by alternative splicing.
Protocols
Product Specific Protocols | |
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WB protocol for PLK4 antibody 12952-1-AP | Download protocol |
IHC protocol for PLK4 antibody 12952-1-AP | Download protocol |
IF protocol for PLK4 antibody 12952-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancers (Basel) POLQ Overexpression Is Associated with an Increased Somatic Mutation Load and PLK4 Overexpression in Lung Adenocarcinoma. | ||
Eur Rev Med Pharmacol Sci LncRNA SNHG1 contributes to tumorigenesis and mechanism by targeting miR-338-3p to regulate PLK4 in human neuroblastoma. | ||
Int J Mol Med MicroRNA‑126 suppresses the proliferation and migration of endothelial cells in experimental diabetic retinopathy by targeting polo‑like kinase 4. | ||
Int J Oncol High PLK4 expression promotes tumor progression and induces epithelial‑mesenchymal transition by regulating the Wnt/β‑catenin signaling pathway in colorectal cancer. | ||
Front Mol Biosci Characteristic Analysis of Featured Genes Associated With Stemness Indices in Colorectal Cancer. | ||
Mol Cell Biol S6 Kinase- and β-TrCP2-Dependent Degradation of p19Arf Is Required for Cell Proliferation. |