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WB analysis of A549 cells using 12952-1-AP
WB result of PLK4 antibody (12952-1-AP, 1:2000) with si-control and si-PLK4 transfected A549 cells.
WB analysis using 12952-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 12952-1-AP (PLK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 12952-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 12952-1-AP (PLK4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12952-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12952-1-AP (PLK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 12952-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12952-1-AP (PLK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 12952-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12952-1-AP (PLK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 12952-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 12952-1-AP (PLK4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 12952-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 12952-1-AP (PLK4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human cervical cancer using 12952-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12952-1-AP (PLK4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human cervical cancer using 12952-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12952-1-AP (PLK4 antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

PLK4 Polyclonal antibody

Name: PLK4 Polyclonal antibody
Brand: Proteintech
SKU: 12952-1-AP

PLK4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12952-1-AP

Publications(25)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

PLK 4, PLK4, Polo like kinase 4, SAK, STK18

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (25)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse lung tissue, MCF-7 cells, A549 cells
Positive IHC detected in	mouse testis tissue, human lung cancer tissue, human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 16 publications below
IHC	See 11 publications below
IF	See 6 publications below

Product Information

12952-1-AP targets PLK4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PLK4 fusion protein Ag3605 Predict reactive species
Full Name	polo-like kinase 4 (Drosophila)
Calculated Molecular Weight	970 aa, 109 kDa
Observed Molecular Weight	105-112 kDa
GenBank Accession Number	BC036023
Gene Symbol	PLK4
Gene ID (NCBI)	10733
RRID	AB_2284150
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O00444
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLK4(Polo-like kinase 4) is also named SAK, STK18 and belongs to the Ser/Thr protein kinase family. It plays a central role in centriole duplication and is a kinase implicated in tumor development, which is an upstream regulator of canonical biogenesis necessary for centriole formation. It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PLK4 antibody 12952-1-AP	Download protocol
IHC protocol for PLK4 antibody 12952-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse, human	WB, IHC	Nat Commun Loss of KLF14 triggers centrosome amplification and tumorigenesis. Authors - Guangjian Fan KO Validated View Article
human	IF	Cancer Res Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification. Authors - Bingteng Xie View Article
mouse	IF	EMBO Rep ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis. Authors - Ting Song View Article
human	WB,IHC	Cell Death Dis Polo-like kinase 4 mediates epithelial-mesenchymal transition in neuroblastoma via PI3K/Akt signaling pathway. Authors - Xiangdong Tian KD Validated View Article
human	WB	Cell Death Dis NudC-like protein 2 restrains centriole amplification by stabilizing HERC2. Authors - Min Li View Article
human	WB	Mol Cancer Ther Targeting Polo-like Kinase 1 by a Novel Pyrrole-imidazole Polyamide-Hoechst Conjugate Suppresses Tumor Growth in vivo. Authors - Ke Liu View Article