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PML Polyclonal antibody
PML Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 21041-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 11 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
Product Information
21041-1-AP targets PML in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PML fusion protein Ag15377 |
| Full Name | promyelocytic leukemia |
| Calculated Molecular Weight | 882 aa, 98 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC020994 |
| Gene Symbol | PML |
| Gene ID (NCBI) | 5371 |
| RRID | AB_2878799 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PML, also named as MYL, RNF71 and TRIM19, is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. It functions as tumor suppressor. PML is required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. It plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. PML plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. It regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitinylation. A large number of alternative spliced transcripts are synthesized from the PML gene, resulting in a variety of PML proteins ranging in molecular weight from 48-97 kDa (PMID:11704850). And it can be detected as 70-130 kDa or larger due to the modification (especially SUMO) (PMID: 16778193, 22438555).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PML antibody 21041-1-AP | Download protocol |
| IHC protocol for PML antibody 21041-1-AP | Download protocol |
| IF protocol for PML antibody 21041-1-AP | Download protocol |
| IP protocol for PML antibody 21041-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res mRNAs are sorted for export or degradation before passing through nuclear speckles. | ||
Theranostics Autophagic degradation of PML promotes susceptibility to HSV-1 by stress-induced corticosterone. | ||
Front Oncol Identification of a Two-Gene (PML-EPB41) Signature With Independent Prognostic Value in Osteosarcoma. | ||
J Ginseng Res 20(S)-ginsenoside Rh2 induces caspase-dependent promyelocytic leukemia-retinoic acid receptor A degradation in NB4 cells via Akt/Bax/caspase9 and TNF-α/caspase8 signaling cascades. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tatyana (Verified Customer) (03-11-2023) | The antibody works really well in ICC in human cells (in 5% goat serum in PBST, ON incubation).
 |
FH Eishi (Verified Customer) (11-17-2017) | Dear colleagues,find attached the WB results. Experimental conditions:Whole protein extraction from mouse embryonic stem cells 25ug protein/laneTransfer 1h30min, 100V, 4C1AB incubation: o/n 4C, 1:500 dilution in milk2AB incubation: 1h RT, 1:20000 dilution in milkDevelopment with femtoThe observed MW is much higher than the expected, with a strong band around 200KDa. This raises the question if the antibody binds specifically to our protein of interest
 |
